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[学术文献 ] Overexpression of StTCP10 Alters Tuber Number and Size in Potato (Solanum tuberosum L.) 进入全文
Plants
Potato (Solanum tuberosum L.), cultivated worldwide for its nutrient-rich underground tubers, represents a crucial staple crop whose yield is primarily determined by both tuber number and tuber size. TCP transcription factors, especially TCP containing miR319 binding sites, play pivotal roles in plant growth and development, yet their functions in potato tuber number and size remain largely unexplored. In this study, we systematically identified 32 TCP genes in potato harboring the conserved TCP domain, among which six were predicted to contain binding sites for Stu-miR319. Semi-quantitative experiments revealed that StTCP10 exhibited the highest expression levels in stolons, swollen stolons, and tuber tissues compared to other StTCP genes containing miR319 binding sites. To elucidate its biological function, we generated StTCP10-overexpressing transgenic potato lines through Agrobacterium-mediated genetic transformation. Phenotypic analysis demonstrated that overexpression of StTCP10 reduced tuber number per plant while enhancing tuber size, with no significant change in total yield. These findings reveal that StTCP10 with Stu-miR319 binding sites plays a critical role in tuber size and mediates the trade-off between tuber size and number, providing novel insights into the molecular breeding aimed at improving tuber size.
[前沿资讯 ] Ansa Biotechnologies Breaks Traditional Synthetic DNA Length Barriers and Redefines Future of Life Science Research 进入全文
Ansa Biotechnologies
"Ansa Biotechnologies, Inc., the trusted partner for DNA synthesis, announced the launch of its 50 kb DNA synthesis early access program, providing customers with extremely long and complex DNA sequences in less than four weeks. DNA products of this length are essential for applications including synthetic genomics, metabolic engineering, agricultural research, and next-generation cell and gene therapies. Ansa will share more information about the early access program and showcase its ultra-rapid and highly accurate enzymatic DNA synthesis technology in booth #312 at the Global Synthetic Biology (SynBioBeta) Conference this week in San Jose, Calif. Ansa’s 50 kb DNA synthesis early access program builds on recent successes and sets a new industry benchmark for synthetic DNA delivery. Researchers can now order large constructs ranging from 7.5 kb to 50 kb. The program is designed to break traditional DNA length barriers, accelerate project timelines, and unlock transformative possibilities in many areas of life science research. “For decades, scientists have been handcuffed by short DNA fragments, forced to stitch sequences together in their own labs through tedious cloning techniques,” said Jason T. Gammack, CEO of Ansa Biotechnologies. “Ansa is now changing the game by delivering longer, more complex DNA, all with a transparent and collaborative service model. This rapid increase in our product length — from only 600 bases a year ago to 7.5 kb more recently and now 50 kb through early access — illustrates our tireless dedication to empowering researchers so they can build without limits and innovate without compromise.” Ansa’s synthesis services are powered by its proprietary enzymatic manufacturing process, which enables the direct synthesis of complex DNA sequences that are often difficult or even impossible to produce with legacy synthesis methods. The sequence-agnostic platform synthesizes DNA strands up to 750 bases long, which are then rapidly assembled into plasmids up to 50 kb. Once synthesized and assembled, constructs are verified using highly accurate long-read sequencing and a proprietary informatics pipeline to ensure that customers receive the exact DNA they ordered. Unlike traditional chemical synthesis, Ansa’s enzymatic approach uses no harsh chemicals, avoiding damage to the DNA molecule and enabling the synthesis of longer, more complex sequences. Entirely manufactured in the USA, Ansa’s DNA products are easy to integrate into any experimental workflow — and are ready to power the next wave of biological innovation. For more information about Ansa’s products, please visit"
[前沿资讯 ] BioLumic Lighting Up Seed Innovation 进入全文
seedworld
BioLumic, a 2025 runner-up in the Seed World Global Innovation Showdown, has created a groundbreaking seed trait platform powered by light. Its technology, which activates traits using short, targeted bursts of UV and visible light, is not only transforming how seeds grow — it’s transforming how seed companies think about innovation. “We’re controlling gene expression with light,” Wargent explains. “We’re activating entire pathways that already exist in the plant, and we’re doing it instantly. There’s no gene editing, no chemicals, and no regulatory delay.” This approach, known as xTrait™ (short for Genetic Expression Trait) technology, taps into photomorphogenesis — plants’ natural response to light that drives development throughout the lifecycle. With Light Signal Recipes™, BioLumic uses brief, precise combinations of UV light as a natural programming language for plants that can boost root mass, increase yield, improve plant immunity and even reduce methane emissions in livestock feed crops. These treatments don’t alter DNA. Instead, they awaken what’s already there.
[前沿资讯 ] Harnessing the Power of Pollen Quality for Improved Seed Production and Breeding 进入全文
seedworld
Enter Amphasys, a pioneering company at the forefront of agricultural innovation. Their cutting-edge technology is revolutionizing how breeders and seed producers assess pollen quality, offering a fast, easy-to-use tool that delivers precision insights. This capability is significant, especially as the agricultural landscape grows increasingly competitive and complex. High-quality pollen is indispensable for effective pollination and achieving optimal seed sets. When it comes to hybrid breeding — often a more expensive and resource-intensive endeavor — pollen health can make or break production outcomes. The reality is that abiotic stressors can wreak havoc on pollen viability; even minor disruptions can hinder the crucial journey from anther to stigma. This is where Amphasys’ technology becomes invaluable, providing data that allows breeders to make informed choices about optimizing crop placement and fine-tuning female-to-male ratios. By determining the viability threshold for full seed set, Amphasys empowers seed producers to develop precise production patterns, particularly crucial for managing low-performing pollinators. Furthermore, the technology supports the development of effective storage protocols, ensuring that pollen maintains its viability for as long as necessary. The implications of these advancements are far-reaching. As growers leverage detailed insights into pollen characteristics, they can significantly enhance their decision-making processes, leading to improved seed yields and crop performance. In an era where food security looms as a critical challenge, this knowledge transforms how the agricultural sector approaches production — making it more efficient, sustainable, and responsive to environmental demands.
[前沿资讯 ] The Future of Potatoes: True Seeds, Hybrid Breeding and a New Commercial Era 进入全文
seedworld
Today, Solynta is entering a bold new chapter: moving from innovation to implementation, from research to real-world impact. Solynta has developed a proprietary hybrid breeding platform that brings precision, speed, and resilience to potato cultivation. Its hybrid TPS varieties offer consistent performance, higher yields, and robust resistance to key threats like late blight, drought, and heat. This level of genetic control has long been common in crops like corn and tomatoes — but achieving it in potatoes is a significant scientific milestone. Now, with a strong foundation of elite parental lines and a growing pipeline of hybrids, Solynta is shifting gears to commercialize its innovations at scale. We have identified several essential resistance genes, and incorporated those in our breeding activities,” said Edwin van der Vossen, Solynta head of research & development. “Many of our newest NON-GMO varieties now have a very high tolerance to late blight. Currently, we are continuing to focus on new traits that will provide additional beneficial characteristics to the future potato varieties. That is the big advantage of our non-GMO hybrid potato breeding technology: we can quickly breed new varieties, very focused and data-driven. Our focus will be on developing new varieties, that are tailored to specific regional climatical zones and fit the market demands”.Peter Poortinga, Solynta CEO. Photo: Solynta. TPS also offers flexibility in how it’s used: direct sowing, transplanting seedlings, or replanting harvested tubers, depending on local needs. This adaptability makes Solynta’s technology suitable for a wide range of environments — from large-scale commercial operations to smallholder farms.
[学术文献 ] Expediting genome synthesis of Corynebacterium glutamicum with an artificial chromosome vector 进入全文
Trends in Biotechnology
Recent advances in genome synthesis have relied on scalable DNA assembly and delivery, and efficient recombination techniques. While these methods have enabled rapid progress for Escherichia coli and yeast, they are often inadequate for other microorganisms. Here, we devised a Corynebacterium glutamicum artificial chromosome (CAC), which combines a replicating system from a closely related strain with an innate partitioning system. This CAC vector can efficiently deliver DNA fragments up to 56 kb and maintain stability in C. glutamicum. Leveraging the CAC vector, we developed CAC Excision Enhanced Recombination (CACEXER), a streamlined strategy for iterative genome replacements in C. glutamicum. Using this approach, we integrated 361 kb (11%) of synthetic DNA into the genome, creating semi-synCG-A. This strain paves the way to establish C. glutamicum as the third industrial microorganism, alongside E. coli and Saccharomyces cerevisiae, to undergo large-scale genome synthesis.
