Reprogramming yeast metabolism to Alter fatty acid profiles from even-chain to odd-chain Configuration

• 全文链接

重新编程酵母代谢以改变脂肪酸谱从偶链到奇链配置

关键词：

来源：

Bioresource Technology

全文链接：

//agri.nais.net.cn/topic/downloadFile/4a40ab07-026c-4776-a04f-c3c6192fd717

来源地址：

https://www.sciencedirect.com/science/article/pii/S0960852425008247?via%3Dihub

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-06-25

摘要：

Odd-chain fatty acids have significant applications in biofuels and pharmaceutical industries. In this study, a yeast cell factory was engineered to produce odd-chain fatty acids and their derivatives. The threonine biosynthesis pathway was initially engineered to enable the de novo synthesis of odd-chain fatty acids, resulting in odd-chain fatty acids accounting for 24.7 % of the total fatty acids. Subsequently, silencing the native fatty acid synthase and introducing a fatty acid synthase from Rhodotorula toruloides, which exhibits higher affinity for propionyl-CoA than the native enzyme, increased the proportion of odd-chain fatty acids to 51.9 %. Further modifications to the lipid metabolism enabled the production of odd-chain free fatty acids (184.1  mg/L) and odd-chain triglycerides (75.2  mg/g). This study successfully shifted the metabolism of Saccharomyces cerevisiae from traditional even-chain fatty acids to a strain dominant in odd-chain fatty acids, demonstrating the potential to develop a novel platform strain for producing specific odd-chain fatty acids derivatives.