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[前沿资讯 ] Ansa Biotechnologies Breaks Traditional Synthetic DNA Length Barriers and Redefines Future of Life Science Research 进入全文
Ansa Biotechnologies
"Ansa Biotechnologies, Inc., the trusted partner for DNA synthesis, announced the launch of its 50 kb DNA synthesis early access program, providing customers with extremely long and complex DNA sequences in less than four weeks. DNA products of this length are essential for applications including synthetic genomics, metabolic engineering, agricultural research, and next-generation cell and gene therapies. Ansa will share more information about the early access program and showcase its ultra-rapid and highly accurate enzymatic DNA synthesis technology in booth #312 at the Global Synthetic Biology (SynBioBeta) Conference this week in San Jose, Calif. Ansa’s 50 kb DNA synthesis early access program builds on recent successes and sets a new industry benchmark for synthetic DNA delivery. Researchers can now order large constructs ranging from 7.5 kb to 50 kb. The program is designed to break traditional DNA length barriers, accelerate project timelines, and unlock transformative possibilities in many areas of life science research. “For decades, scientists have been handcuffed by short DNA fragments, forced to stitch sequences together in their own labs through tedious cloning techniques,” said Jason T. Gammack, CEO of Ansa Biotechnologies. “Ansa is now changing the game by delivering longer, more complex DNA, all with a transparent and collaborative service model. This rapid increase in our product length — from only 600 bases a year ago to 7.5 kb more recently and now 50 kb through early access — illustrates our tireless dedication to empowering researchers so they can build without limits and innovate without compromise.” Ansa’s synthesis services are powered by its proprietary enzymatic manufacturing process, which enables the direct synthesis of complex DNA sequences that are often difficult or even impossible to produce with legacy synthesis methods. The sequence-agnostic platform synthesizes DNA strands up to 750 bases long, which are then rapidly assembled into plasmids up to 50 kb. Once synthesized and assembled, constructs are verified using highly accurate long-read sequencing and a proprietary informatics pipeline to ensure that customers receive the exact DNA they ordered. Unlike traditional chemical synthesis, Ansa’s enzymatic approach uses no harsh chemicals, avoiding damage to the DNA molecule and enabling the synthesis of longer, more complex sequences. Entirely manufactured in the USA, Ansa’s DNA products are easy to integrate into any experimental workflow — and are ready to power the next wave of biological innovation. For more information about Ansa’s products, please visit"
[学术文献 ] Expediting genome synthesis of Corynebacterium glutamicum with an artificial chromosome vector 进入全文
Trends in Biotechnology
Recent advances in genome synthesis have relied on scalable DNA assembly and delivery, and efficient recombination techniques. While these methods have enabled rapid progress for Escherichia coli and yeast, they are often inadequate for other microorganisms. Here, we devised a Corynebacterium glutamicum artificial chromosome (CAC), which combines a replicating system from a closely related strain with an innate partitioning system. This CAC vector can efficiently deliver DNA fragments up to 56 kb and maintain stability in C. glutamicum. Leveraging the CAC vector, we developed CAC Excision Enhanced Recombination (CACEXER), a streamlined strategy for iterative genome replacements in C. glutamicum. Using this approach, we integrated 361 kb (11%) of synthetic DNA into the genome, creating semi-synCG-A. This strain paves the way to establish C. glutamicum as the third industrial microorganism, alongside E. coli and Saccharomyces cerevisiae, to undergo large-scale genome synthesis.
[学术文献 ] The phased pan-genome of tetraploid European potato 进入全文
Nature
Potatoes were first brought to Europe in the sixteenth century. Two hundred years later, one of the species had become one of the most important food sources across the entire continent and, later, even the entire world. However, its highly heterozygous, autotetraploid genome has complicated its improvement since then. Here we present the pan-genome of European potatoes generated from phased genome assemblies of ten historical potato cultivars, which includes approximately 85% of all haplotypes segregating in Europe. Sequence diversity between the haplotypes was extremely high (for example, 20× higher than in humans), owing to numerous introgressions from wild potato species. By contrast, haplotype diversity was very low, in agreement with the population bottlenecks caused by domestication and transition to Europe. To illustrate a practical application of the pan-genome, we converted it into a haplotype graph and used it to generate phased, megabase-scale pseudo-genome assemblies of commercial potatoes (including the famous French fries potato ‘Russet Burbank’) using cost-efficient short reads only. In summary, we present a nearly complete pan-genome of autotetraploid European potato, we describe extraordinarily high sequence diversity in a domesticated crop, and we outline how this resource might be used to accelerate genomics-assisted breeding and research.
[前沿资讯 ] 江苏多地做强专业园区促进企业集聚 期待合成生物产业“合成”更多惊喜 进入全文
江苏省人民政府
3月31日，2025第三届合成生物学产业博览会在南京江北新区举办，展会热度很高，吸引了近百家参展方。近年来，我国高度重视生物制造产业发展，合成生物技术作为核心驱动力，已成为推进制造强国建设的重要抓手。江苏多地积极布局合成生物产业园区，如长三角合成生物产业创新园等，推动产业集聚。同时，合成生物技术在医疗健康、食品、农业等领域应用广泛，吸引了众多企业进入。聚焦生物医药、食品、生物基材料等方向，强化产业源头技术创新，支持相关中心及高校院所布局合成生物学研究，突破生物制造核心技术。
[学术文献 ] Colony pattern multistability emerges from a bistable switch 进入全文
PNAS
Microbial colony development hinges upon a myriad of factors, including mechanical, biochemical, and environmental niches, which collectively shape spatial patterns governed by intricate gene regulatory networks. The inherent complexity of this phenomenon necessitates innovative approaches to comprehend and compare the mechanisms driving pattern formation. Here, we unveil the multistability of bacterial colony patterns, where bacterial colony patterns can stabilize into multiple distinct types including ring-like patterns and sector-like patterns on hard agar, orchestrated by a simple synthetic bistable switch. Utilizing quantitative imaging and spatially resolved transcriptome approaches, we explore the deterministic process of a ring-like colony pattern formation from a single cell. This process is primarily driven by bifurcation events programmed by the gene regulatory network and microenvironmental cues. Additionally, we observe a noise-induced process amplified by the founder effect, leading to patterns of symmetry-break during range expansion. The degrees of asymmetry are profoundly influenced by the initial conditions of single progenitor cells during the nascent stages of colony development. These findings underscore how the process of range expansion enables individual cells, exposed to a uniform growth-promoting environment, to exhibit inherent capabilities in generating emergent, self-organized behavior.
[政策法规 ] 韩国通过合成生物学促进法案 进入全文
韩联社
"韩国国会日前表决通过《合成生物学促进法案》，将合成生物学确立为国家核心技术并制定专项培育政策。该法案由韩国科学技术信息通信部主导推进，旨在通过系统性政策支持提升韩国在该领域的全球竞争力，抢占未来生物技术制高点。 合成生物学核心在于对基因、蛋白质等生物元件进行标准化重组，形成“生物积木”式创新应用，如汽车组装般构建新型生命系统。按照韩国国家科学技术咨询委员会的评估，韩国当前合成生物学竞争力位列全球第七。此次通过的法案要求韩国科学技术信息通信部每五年制定国家基本计划，设立专项政策机构与研发基地，构建“政—研—产”协同创新体系。韩国科学技术信息通信部表示，该法案为韩国把握生物经济革命性机遇提供了国家战略框架。该法案的通过将推动总投资1263亿韩元的国家级“生物铸造工厂”建设。该设施借鉴半导体晶圆厂模式，通过AI技术实现生物元件设计—构建—测试的全流程自动化，可将生物元件开发周期缩短80%。 韩国政府表示，将聚焦合成生物学引发的全球生物市场范式变革，重点突破基因编辑、人工细胞设计等关键技术，目标在2030年前跻身全球前五大技术强国。韩国业界分析指出，随着法案实施，韩国生物医药、生物制造、碳中和等战略产业将获得更多底层技术支撑。"
