Targeting lignocellulolytic gene clusters in novel Trichoderma atroviride and Trichoderma harzianum strains through bacterial artificial chromosome–guided analysis

• 全文链接

通过细菌人工染色体引导分析靶向新型阿曲韦利木霉和哈氏木霉菌株中的木质纤维素分解基因簇

关键词：

来源：

Mycologia

全文链接：

//agri.nais.net.cn/topic/downloadFile/8ac10441-7bb2-4cdb-b3ea-d72082b3445a

来源地址：

https://www.tandfonline.com/doi/full/10.1080/00275514.2025.2496600

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-06-27

摘要：

Lignocellulosic biomass is a complex carbon source with recalcitrant properties whose degradation via industrial enzymatic hydrolysis is challenging, directly affecting the cost of reliable energy production. In nature, filamentous fungi, including Trichoderma species, degrade lignocellulose via an arsenal of hydrolytic and oxidative enzymes that act synergistically to process it into soluble sugar monomers. This work explored the genomic content of Trichoderma atroviride and Trichoderma harzianum strains with hydrolytic abilities by identifying regions possessing degradative enzyme–encoding genes, namely, hydrolytic clusters. We employed bacterial artificial chromosome (BAC) methodology to target specific genomic regions and explore their genetic organization, proximal gene context, and gene expression under degradative conditions. With this tool, it was possible to inspect the linear structure and expression profile of target hydrolytic-rich genomic regions. The present work offers a perspective on the organization of genome regions related to carbohydrate metabolism. This study revealed novel genes and genome regions that are positively regulated during cellulose degradation, contributing to elucidating differences in gene organization that potentially impact hydrolysis among Trichoderma species.