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[学术文献 ] Molecular Characterization and Functional Analysis of Odorant-Binding Proteins in Ectropis grisescens 进入全文
International journal of molecular sciences 期刊
Insect odorant-binding proteins (OBPs) are promising molecular targets for developing novel pest management strategies by modulating chemoreception-driven behaviors. The tea gray geometrid Ectropis grisescens (Lepidoptera, Geometridae) is a major pest in tea plantations, causing substantial economic losses in China. In this study, we identified 18 OBPs from E. grisescens antennal transcriptome. All of the encoded proteins possessed N-terminal signal peptides and conserved cysteine residues, behaviors which are characteristic of insect OBPs. Phylogenetic analysis categorized these proteins into plus-C, minus-C, and classic OBP subfamilies. MEME motif analysis identified conserved sequence features potentially involved in odor detection. Tissue- and sex-specific expression profiling showed that EgriGOBP1-2, OBP3, OBP8, and OBP13 were highly expressed in the antennae of both sexes, suggesting roles in olfactory communication. Among them, EgriGOBP1-2, OBP3, and OBP13 exhibited similar expression levels between males and females, while other EgriOBPs were predominantly expressed in the legs, wings, or other tissues, indicating additional physiological functions beyond chemoreception. To investigate functional specificity, we selected antenna-enriched EgriGOBP2 for ligand-binding analysis. Fluorescence binding assays demonstrated that EgriGOBP2 exhibited broad binding affinity toward 8 of 12 host volatiles and 11 of 12 plant essential oil-derived volatiles. These combined findings lay the foundation for mechanistic studies of chemical recognition in E. grisescens and provide insights into the development of ecologically friendly pest control alternatives.
[学术文献 ] CsUGT95B11 glycosylates quercetin to enhance resistance of tea plant (Camellia sinensis) to Ectropis grisescens 进入全文
Plant Physiology and Biochemistry 期刊
Quercetin is one of the major flavonols of tea plants, which was glycosylated to involve responses to various environmental stresses. However, not much is known about the biosynthesis of glycosylated quercetin derivatives in tea plant in response to herbivorous attack. Here, we found that E. grisescens attack significantly increased the contents of quercetin 3-O-glucoside and quercetin 7-O-glucoside in tea leaves, and exogenous application of these two compounds improved the E. grisescens resistance of tea plant. Integrated transcriptome and phylogenetic analyses identified CsUGT95B11 gene, encoding a quercetin glycosyltransferase, which was upregulated in response to E. grisescens attack. Enzymatic assays showed that CsUGT95B11 preferentially glycosylated quercetin to produce quercetin 7-O-glucoside with a kcat·Km−1 value of 9655.52 M−1 s−1. Subcellular localization analysis indicated that CsUGT95B11 was dispersed in the cytoplasm of Nicotiana benthamiana leaves and Arabidopsis protoplast. Suppression of CsUGT95B11 reduced the quercetin glucoside content of tea leaves and impaired resistance of tea plant to E. grisescens. These results indicate that CsUGT95B11 is a quercetin glycosyltransferase that enhances resistance of tea plant to E. grisescens, providing insights into the mechanism of flavonol glycosylation regulating interactions between plants and herbivores.
[学术文献 ] Comparison of the effect of tea shoots during different seasons in Arma chinensis (Hemiptera: Pentatomidae) reared on Ectropis grisescens (Lepidoptera: Geometridae) pupae 进入全文
Journal of Economic Entomology 期刊
In this study, we compared the growth, development, and fecundity of Arma chinensis (Fallou) reared on pupae of the geometrid Ectropis grisescens Warren fed on tea shoots during different seasons of the year. The raw data on life history were analyzed using the age–stage, 2-sex life table. When reared on spring or winter geometrid pupae, the duration of the immature stage of A. chinensis was significantly longer than in those produced during the summer or autumn. The survival rate of immature A. chinensis reared on autumn geometrid pupae was significantly lower compared to other treatments. Reproductive diapause was observed in adult A. chinensis reared on winter geometrid pupae. The adult preoviposition period (APOP), total preoviposition period (TPOP), and total longevity were significantly longer in A. chinensis reared on winter pupae than in the other treatments. The fecundity of A. chinensis reared on spring geometrid pupae was significantly lower than in the other treatments. The higher intrinsic rate of increase of the A. chinensis reared on summer pupae (r = 0.0966 day-1) and autumn pupae (r = 0.0983 day-1) resulted in higher fecundity, shorter immature duration, and shorter TPOP compared to the winter and spring populations. These findings can be utilized to enhance and sustain biological control of E. grisescens in tea plantations.
[学术文献 ] Impact of Ectropis grisescens Warren (Lepidoptera: Geometridae) Infestation on the Tea Plant Rhizosphere Microbiome and Its Potential for Enhanced Biocontrol and Plant Health Management 进入全文
Insects 期刊
The study took samples of leaves, roots, and rhizosphere soil at different times after the plants were attacked by E. grisescens. Using 16S rRNA sequencing, we observed significant shifts in bacterial communities by the seventh day. Transcriptomic analysis showed that the E. grisescens attack triggered reprogramming of the tea root transcriptome, leading to changes in the rhizosphere bacterial community. Analysis of the rhizosphere soil on the seventh day revealed alterations in the microbial network structure and the core microorganisms within the network following the attack; microorganisms related to nitrogen (N) metabolism were mobilized in the rhizosphere. Notably, nitrogen-fixing bacteria from the genus Burkholderia were actively recruited and became important contributors in the rhizosphere. When these separated Burkholderia strains were reintroduced into tea trees, the level of insect-resistant substances in tea leaves was increased, thereby improving the insect resistance of tea trees.
[统计数据 ] Tea Area Harvested in World and Five Continents in 2023 (FAOSTAT) 进入全文
FAO 网站
根据FAOSTAT，最新统计了2023年度全球及五大洲（包括：非洲、美洲、亚洲、欧洲、大洋洲和世界）的茶叶种植面积，数据详见表Tea Area Harvested in World and Five Continents in 2023（FAOSTAT）。
[会议论文 ] Monitoring the Daily Rhythm of Total Green Leaf Volatiles with a Low-Cost Multi-Sensor Node 进入全文
IEEE 会议
Plants emit volatile organic compounds (VOCs) from their leaves, flowers or fruits. These chemical signals have many functions ranging from hormonal regulation to complex interactions with pollinators and herbivores. Even diseases can influence the emission of VOCs. Thus, VOC monitoring might allow to deduce the abiotic and biotic stress of plants and to support data based decisions on countermeasures. However, most sensing methods for VOCs are not low-cost and mobile, which hinders widespread application. We report here on the possibility of employing off-the-shelf air quality sensors for sensing VOC levels emitted by plants. The developed low-cost multi-sensor node is described in detail. As a proof of principle, we monitor the VOC emission from the subshrub rue and show that a cyclic day-night rhythm of total VOC emission can be observed.
