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[学术文献 ] A Golgi vesicle-membrane-localized cytochrome B561 regulates ascorbic acid regeneration and confers Verticillium wilt resistance in cotton 进入全文
PLANT JOURNAL
Ascorbic acid (AsA) serves as a key antioxidant involved in the various physiological processes and against diverse stresses in plants. Due to the insufficiency of AsA de novo biosynthesis, the AsA regeneration is essential to supplement low AsA synthesis rates. Redox reactions play a crucial role in response to biotic stress in plants; however, how AsA regeneration participates in hydrogen peroxide (H2O2) homeostasis and plant defense remains largely unknown. Here, we identified a Golgi vesicle-membrane-localized cytochrome B561 (CytB561) encoding gene, GhB561-11, involved in AsA regeneration and plant resistance to Verticillium dahliae in cotton. GhB561-11 was significantly downregulated upon V. dahliae attack. Knocking down GhB561-11 greatly enhanced cotton resistance to V. dahliae. We found that suppressing GhB561-11 inhibited the AsA regeneration, elevated the basal level of H2O2, and enhanced the plant defense against V. dahliae. Further investigation revealed that GhB561-11 interacted with the lipid droplet-associated protein GhLDAP3 to collectively regulate the AsA regeneration. Simultaneously silencing GhB561-11 and GhLDAP3 significantly elevated the H2O2 contents and dramatically improved the Verticillium wilt resistance in cotton. The study broadens our insights into the functional roles of CytB561 in regulating AsA regeneration and H2O2 homeostasis. It also provides a strategy by downregulating GhB561-11 to enhance Verticillium wilt resistance in cotton breeding programs.
[学术文献 ] Utilizing the mutant library to investigate the functional characterization of GhGLR3.4 regulating jasmonic acid to defense pest infestation 进入全文
PLANT JOURNAL
The glutamate receptor (GLR) serves as a ligand-gated ion channel that plays a vital role in plant growth, development, and stress response. Nevertheless, research on GLRs in cotton is still very limited. The present study conducted a comprehensive analysis of GLRs gene family in cotton. In total, 41 members of the GLR family were identified in cotton unveiling distinct subgroups in comparison to Arabidopsis. Among these members, the third subgroup highlights its pivotal role in cotton's defense against insect infestation. Furthermore, the CRISPR/Cas9 system was utilized to create a mutant library of GLR members, which consisted of a total of 135 independent mutant lines, resulting in the production of novel cotton materials with valuable breeding potential for pest control. Further, this study elucidates the influence of GhGLR3.4 on jasmonic acid (JA) pathway signal transduction and demonstrated its participation in the influx of intracellular Ca2+, which regulates "calcium transients" following stimulation, thereby influencing multiple intracellular reactions. The study also found that GhGLR3.4 influences the synthesis of the JA pathway and actively partakes in long-distance signal transmission among plants, facilitating the transfer of defense signals to neighbor leaves and thereby triggering systemic defense. Consequently, this research advances our knowledge of plants' comprehensive defense mechanism against insect pest infestation.
[学术文献 ] GhTBL3 is required for fiber secondary cell wall (SCW) formation via maintaining acetylation of xylan in cotton 进入全文
PLANT JOURNAL
TBL family proteins containing the domain of unknown function mainly act as xylan O-acetyltransferases, but the specific molecular mechanism of their functions remains unclear in plants (especially in cotton) so far. In this study, we characterized the TBL family proteins containing the conserved GDS and DxxH motifs in cotton (Gossypium hirsutum). Among them, GhTBL3 is highly expressed in fibers at the stage of secondary cell wall (SCW) formation and mainly functions as O-acetyltransferase to maintain acetylation of xylan in fiber SCW development. Overexpression of GhTBL3 in cotton promoted fiber SCW formation, resulting in increased fiber cell wall thickness. In contrast, suppression of GhTBL3 expression in cotton impaired fiber SCW synthesis, leading to the decreased fiber cell wall thickness, compared with wild type (WT). Furthermore, two fiber SCW-related transcription factors GhMYBL1 and GhKNL1 were found to directly bind to the promoter of GhTBL3 in cotton. GhMYBL1 enhanced the transcription activity of GhTBL3, whereas GhKNL1 inhibited the expression of GhTBL3 in fibers. The acetylation level of xylan was remarkably decreased in fibers of GhMYBL1 RNAi transgenic cotton, but the acetylation level of xylan was significantly increased in fibers of GhKNL1 RNAi cotton, relative to WT. Given together, the above results suggested that GhTBL3 may be under the dual control of GhMYBL1 and GhKNL1 to maintain the suitable acetylation level of xylan required for fiber SCW formation in cotton. Thus, our data provide an effective clue for potentially improving fiber quality by genetic manipulation of GhTBL3 in cotton breeding.
[学术文献 ] Re-localization of a repeat-containing fungal effector by apoplastic protein Chitinase-like 1 blocks its toxicity 进入全文
NATURE COMMUNICATIONS
A fungal effector that is toxic to plant cells was identified in Verticillium dahliae. The effector contains a non-canonical Common in several Fungal Extracellular Membrane proteins (CFEM) domain, a tandem repeat region consisting of four 14-amino acid repeats rich in proline, and a C-terminal region, thus is designated V. dahliae tetrapeptide repeat protein (VdTRP). The membrane targeting of VdTRP is vital for its cell toxicity. CFEM mediates the membrane targeting and the tandem repeat region exerts the toxic function upon cell membrane. The chitinase-like 1 (CTL1), an essential apoplastic protein of cotton, can redirect VdTRP from cell membrane to apoplast. Transgenic cotton overexpressing CTL1 greatly enhances cotton resistance to V. dahliae without affecting cotton growth and development, implicating its potential application in breeding cotton with high wilt resistance. Our data demonstrates that genetic manipulation of effector target constitutes potential strategy for improving crop resistance to fungal pathogens. A toxin identified in Verticillium dahliae, VdTRP, damages cell membranes and causes rapid cell death. As defense, cotton uses chitinase-like 1, a cell wall protein, to keep VdTRP in the apoplastic space and reduce its toxicity to plant cells.
[学术文献 ] Environmental impacts of genetically modified crops 进入全文
SCIENCE
Genetically modified (GM) crops have been adopted by some of the world's leading agricultural nations, but the full extent of their environmental impact remains largely unknown. Although concerns regarding the direct environmental effects of GM crops have declined, GM crops have led to indirect changes in agricultural practices, including pesticide use, agricultural expansion, and cropping patterns, with profound environmental implications. Recent studies paint a nuanced picture of these environmental impacts, with mixed effects of GM crop adoption on biodiversity, deforestation, and human health that vary with the GM trait and geographic scale. New GM or gene-edited crops with different traits would likely have different environmental and human health impacts.
[学术文献 ] Scalable multifunctional MOFs-textiles via diazonium chemistry 进入全文
NATURE COMMUNICATIONS
Cellulose fiber-based textiles are ubiquitous in daily life for their processability, biodegradability, and outstanding flexibility. Integrating cellulose textiles with functional coating materials can unlock their potential functionalities to engage diverse applications. Metal-organic frameworks (MOFs) are ideal candidate materials for such integration, thanks to their unique merits, such as large specific surface area, tunable pore size, and species diversity. However, achieving scalable fabrication of MOFs-textiles with high mechanical durability remains challenging. Here, we report a facile and scalable strategy for direct MOF growth on cotton fibers grafted via the diazonium chemistry. The as-prepared ZIF-67-Cotton textile (ZIF-67-CT) exhibits excellent ultraviolet (UV) resistance and organic contamination degradation via the peroxymonosulfate activation. The ZIF-67-CT is also used to encapsulate essential oils such as carvacrol to enable antibacterial activity against E. coli and S. aureus. Additionally, by directly tethering a hydrophobic molecular layer onto the MOF-coated surface, superhydrophobic ZIF-67-CT is achieved with excellent self-cleaning, antifouling, and oil-water separation performances. More importantly, the reported strategy is generic and applicable to other MOFs and cellulose fiber-based materials, and various large-scale multi-functional MOFs-textiles can be successfully manufactured, resulting in vast applications in wastewater purification, fragrance industry, and outdoor gears. Integrating cellulose textiles with functional coating materials can unlock their potential functionalities to engage diverse applications. Here, authors report a generic strategy to achieve highly stable, large-scale, and multifunctional MOFs-textiles via diazonium chemistry.
