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[前沿资讯 ] NBRI’s innovative chip to boost cotton cultivation 进入全文
THE TIMES OF INDIA
Lucknow: CSIR-National Botanical Research Institute (NBRI), Lucknow, has developed a special chip that will assist scientists and farmers in cultivating superior cotton plants. Upon insertion of this ‘90K SNP Cotton Chip' in special equipment, it will provide data about various cotton varieties and their characteristics. The chip facilitates the development of high-quality cotton plants through marker-assisted breeding (MAB), a DNA-based approach. This utilises molecular markers to identify and choose plants with specific traits, creating new varieties. "The chip contains data of around 90,000 cotton SNP markers, which can be used to crossbreed and create a new variety according to climatic, production or pest control needs. This is the first such chip in India, and its license was given to a Delhi-based company in the presence of CSIR director general N Kalaiselvi," said NBRI director Ajit Kumar Shasany. Explaining MAB or chip technology, Shasany said: "In agricultural production, we often aim to combine good traits from different plants to breed a new variety. Suppose we have a cotton plant with many seeds but fewer branches and is not drought or pest-resistant while another variety has fewer seeds but is drought and pest-resistant and has more branches. We can combine these two to breed a desirable variety." "This may sound easy, but it's a herculean task as suitable varieties must be identified from thousands before crossbreeding. It may take months and even years. It's tough to determine which one is the best. The agricultural performance of plants is usually linked to traits that are encoded by DNA," he said. Shasany added that this chip was prepared by sequencing 320 cotton genotypes found in India, which resulted in 40 lakh single nucleotide polymorphisms (SNP), a variation in the DNA sequence at a single base position. Out of these, 90K SNPs were shortlisted as the best markers. Lucknow: CSIR-National Botanical Research Institute (NBRI), Lucknow, has developed a special chip that will assist scientists and farmers in cultivating superior cotton plants. Upon insertion of this ‘90K SNP Cotton Chip' in special equipment, it will provide data about various cotton varieties and their characteristics. The chip facilitates the development of high-quality cotton plants through marker-assisted breeding (MAB), a DNA-based approach. This utilises molecular markers to identify and choose plants with specific traits, creating new varieties. "The chip contains data of around 90,000 cotton SNP markers, which can be used to crossbreed and create a new variety according to climatic, production or pest control needs. This is the first such chip in India, and its license was given to a Delhi-based company in the presence of CSIR director general N Kalaiselvi," said NBRI director Ajit Kumar Shasany. Explaining MAB or chip technology, Shasany said: "In agricultural production, we often aim to combine good traits from different plants to breed a new variety. Suppose we have a cotton plant with many seeds but fewer branches and is not drought or pest-resistant while another variety has fewer seeds but is drought and pest-resistant and has more branches. We can combine these two to breed a desirable variety." "This may sound easy, but it's a herculean task as suitable varieties must be identified from thousands before crossbreeding. It may take months and even years. It's tough to determine which one is the best. The agricultural performance of plants is usually linked to traits that are encoded by DNA," he said. Shasany added that this chip was prepared by sequencing 320 cotton genotypes found in India, which resulted in 40 lakh single nucleotide polymorphisms (SNP), a variation in the DNA sequence at a single base position. Out of these, 90K SNPs were shortlisted as the best markers.
[学术文献 ] An infrared-transparent textile with high drawing processed Nylon 6 nanofibers 进入全文
NATURE COMMUNICATIONS
Infrared (IR)-transparent radiative cooling textiles show great promise for achieving personal thermal comfort and reducing energy consumption. However, besides a few synthetic fiber materials proposed as IR-transparent textiles, traditional textile materials used to achieve IR transparency have not been realized, impeding large-scale practical applications. Here, based on a common textile material Nylon 6 (PA6), we design a high drawing process with rapid solvent evaporation to achieve IR-transparent PA6 textiles. By altering the chain conformations and crystal structures, the molecular vibrations in the IR region (IR absorption) of PA6 can be significantly weakened. Meanwhile, this process also tailors the fiber to the nanoscale and minimizes IR reflection. Consequently, a human body covered by our textile can stay 2.1 degrees C cooler than with cotton, corresponding to similar to 20% indoor energy savings in cooling. We expect that our work offers an innovative pathway to regulate IR radiation for personal thermal management.
[学术文献 ] PtrVINV2 is dispensable for cellulose synthesis but essential for salt tolerance in Populus trichocarpa Torr. and Gray 进入全文
PLANT BIOTECHNOLOGY JOURNAL
Invertase (EC.3.2.1.26), a key enzyme in sucrose breakdown, is crucial for cellulose synthesis. However, the function of the vacuolar invertase (VINV) in woody plants remains unclear. In this study, transgenic lines of Populus trichocarpa Torr. and Gray were generated to investigate the role of PtrVINV2 in wood formation and under high salinity stress. Compared to wild-type (WT), VINV activity in the developing xylem of knockout lines was reduced, resulting in a decrease in lignin content and an increase in hemicellulose content, while cellulose content remained unaffected. These changes in structural carbohydrate content were accompanied by reductions in xylem width and fibre cell wall thickness. The overexpression lines of the developing xylem exhibited opposite trends. Transcriptome analyses of developing xylem indicated that the expression level of PtrVINV2 affects the expression of genes involved in hemicellulose and lignin biosynthesis pathways, such as AXS, UAMs, HCT, COMT, CAD and peroxidases, while CesA expression remained unaffected. WGCNA analysis revealed that Potri.001G219100, Potri.009G106600 and Potri.002G081000 serve as 'hub' transcription factor genes within the structural/non-structural carbohydrate modules of PtrVINV2 transgenic lines, potentially involved in plant salt tolerance. Additionally, under 200 mmol/L NaCl treatment, the knockout lines exhibited increased salt sensitivity compared to WT. This increased sensitivity was accompanied by elevated activities of SOD, CAT and MDA, as well as higher sucrose content and reduced contents of glucose and fructose. The findings indicate that although PtrVINV2 is not essential for cellulose synthesis, it enhances salt tolerance in poplar and presents a promising candidate gene for breeding salt-tolerant poplar.
[学术文献 ] CYTOKININ DEHYDROGENASE suppression increases intrinsic water-use efficiency and photosynthesis in cotton under drought 进入全文
PLANT PHYSIOLOGY
Drought reduces endogenous cytokinin (CK) content and disturbs plant water balance and photosynthesis. However, the effect of higher endogenous CK levels (achieved by suppressing cytokinin dehydrogenase [CKX] genes) on plant water status and photosynthesis under drought stress is unknown. Here, pot experiments were conducted with wild-type (WT) cotton (Gossypium hirsutum) and 2 GhCKX suppression lines (CR-3 and CR-13) to explore the effect of higher endogenous CK levels on leaf water utilization and photosynthesis under drought stress. The GhCKX suppression lines had a higher leaf net photosynthetic rate (AN) and intrinsic water-use efficiency (iWUE) than WT under drought. This increase was attributed to the decoupling of stomatal conductance (gs) and mesophyll conductance (gm) in the suppression lines in response to drought. GhCKX suppression increased gm but maintained gs relative to WT under drought, and the increased gm was associated with altered anatomical traits, including decreased cell wall thickness (Tcw) and increased surface area of chloroplast-facing intercellular airspaces per unit leaf area (Sc/S), as well as altered cell wall composition, especially decreased cellulose levels. This study provides evidence that increased endogenous CK levels can simultaneously enhance AN and iWUE in cotton under drought conditions and establishes a potential mechanism for this effect. These findings provide a potential strategy for breeding drought-tolerant crops or exploring alternative methods to promote crop drought tolerance. Increasing endogenous cytokinin levels alters anatomical traits related to stomatal and mesophyll conductance, resulting in their decoupling and enhanced drought tolerance in cotton.
[学术文献 ] A cell fractionation and quantitative proteomics pipeline to enable functional analyses of cotton fiber development 进入全文
PLANT JOURNAL
Cotton fibers are aerial trichoblasts that employ a highly polarized diffuse growth mechanism to emerge from the developing ovule epidermis. After executing a complicated morphogenetic program, the cells reach lengths over 2 cm and serve as the foundation of a multi-billion-dollar textile industry. Important traits such as fiber diameter, length, and strength are defined by the growth patterns and cell wall properties of individual cells. At present, the ability to engineer fiber traits is limited by our lack of understanding regarding the primary controls governing the rate, duration, and patterns of cell growth. To gain insights into the compartmentalized functions of proteins in cotton fiber cells, we developed a label-free liquid chromatography mass spectrometry method for systems-level analyses of fiber proteome. Purified fibers from a single locule were used to fractionate the fiber proteome into apoplast (APOT), membrane-associated (p200), and crude cytosolic (s200) fractions. Subsequently, proteins were identified, and their localizations and potential functions were analyzed using combinations of size exclusion chromatography, statistical and bioinformatic analyses. This method had good coverage of the p200 and APOT fractions, the latter of which was dominated by proteins associated with particulate membrane-enclosed compartments. The apoplastic proteome was diverse, the proteins were not degraded, and some displayed distinct multimerization states compared to their cytosolic pool. This quantitative proteomic pipeline can be used to improve coverage and functional analyses of the cotton fiber proteome as a function of developmental time or differing genotypes.
[学术文献 ] GhMYB102 affects cotton fibre elongation and secondary wall thickening by regulating GhIRX10 in cotton 进入全文
PLANT BIOTECHNOLOGY JOURNAL
Upland cotton (Gossypium hirsutum) is a principal economic crop and a fundamental raw material for the textile industry. The quality of cotton fibres is significantly influenced by the synthesis of cell wall polysaccharides. This study focuses on GhIRX10, a beta-1,4-xylosyltransferase crucial for xylan backbone synthesis. Overexpression of GhIRX10 enhances xylan synthesis, which impacts fibre elongation and secondary cell wall thickening. GhMYB102, identified as a direct regulator of GhIRX10 expression, was confirmed through comprehensive validation. Overexpression of GhMYB102 resulted in a similar phenotype as OE-GhIRX10: increased cell wall thickness and reduced fibre length. Overexpression of GhMYB102 upregulated the expression of key cell wall synthesis-related genes, including GhCESA4/7/8, GhIRXs, GhCESAs, GhGUXs, GhTBLs, GhXTHs, and GhXXTs. Consequently, the cellulose and hemicellulose contents in OE-GhMYB102 lines were significantly increased. GhMYB102 was also validated as a target gene regulated by GhFSN1 and GhMYB7, with the ability to reciprocally regulate GhFSN1 expression. In summary, we propose a regulatory model where GhMYB102 promotes the expression of GhIRX10 and other cell wall-related genes, thereby affecting fibre quality. This study elucidates the regulatory network of secondary cell wall synthesis in cotton and provides potential targets for improving fibre quality through molecular breeding.
