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Data from: Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.1rn8pk0qz
- 摘要：
- and multi-trophic level interactions. Both conventional polymerase chain reaction (cPCR) and quantitative PCR (qPCR) have been employed to detect predation
Data from: Attack of the PCR clones: rates of clonality have little effect on RAD-seq genotype calls
- 负责人：
- DOI：
- doi:10.5061/dryad.3mq4631
- 摘要：
- results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site associated sequencing
Data from: PCR duplication: a one-step cloning-free method to generate duplicated chromosomal loci and interference-free expression reporters
- 负责人：
- DOI：
- doi:10.5061/dryad.gj51n
- 摘要：
- Here, we report on a novel PCR targeting-based strategy called ‘PCR duplication’ that enables targeted duplications of genomic region
Data from: Rates of hypoxia induction alter mechanisms of O2 uptake and the critical O2 tension of goldfish
- 负责人：
- DOI：
- doi:10.5061/dryad.bp20p
- 摘要：
- reduce the critical O2 tension (Pcrit) of O2 uptake rate (?O2). We investigated this by determining Pcrit values for goldfish exposed to short (?24 min
Data from: The GPCR repertoire in the demosponge Amphimedon queenslandica: insights into the GPCR system at the early divergence of animals
- 负责人：
- DOI：
- doi:10.5061/dryad.43t7r
- 摘要：
- Background: G protein-coupled receptors (GPCRs) play a central role in eukaryotic signal transduction. However, the GPCR component of this signalling
Data from: Clinical and laboratory predictors of influenza infection among individuals with influenza-like illness presenting to an urban Thai hospital over a five-year period
- 负责人：
- DOI：
- doi:10.5061/dryad.t7n48
- 摘要：
- by RT-PCR) among individuals presenting for care with ILI. Nasal and throat swabs were tested by rapid influenza test (QuickVue) and by RT-PCR
Data from: Collecting in collections: a PCR strategy and primer set for DNA barcoding of decades-old dried museum specimens
- 负责人：
- DOI：
- doi:10.5061/dryad.6v4h5
- 摘要：
- specimens collected specifically for that purpose, instead of the wealth of identified material in museums, constrained by the lack of suitable PCR
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- in vitro specificity tests for 38 species-specific primers selected for seven fishes and six turtles, using singleplex conventional PCR (cPCR). A subset
Data from: Benefits and challenges with applying unique molecular identifiers in next generation sequencing to detect low frequency mutations
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n6068
- 摘要：
- Indexing individual template molecules with a unique identifier (UID) before PCR and deep sequencing is promising for detecting low frequency
Data from: A whole-transcriptome approach to evaluating reference genes for quantitative gene expression studies: a case study in Mimulus
- 负责人：
- DOI：
- doi:10.5061/dryad.84655
- 摘要：
- While quantitative PCR (qPCR) is widely recognized as being among the most accurate methods for quantifying gene expression, it is highly dependent
