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Data from: Modular tagging of amplicons using a single PCR for high-throughput sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.22412
- 摘要：
- High-throughput sequencing (HTS) of PCR amplicons is becoming the method of choice to sequence one or several targeted loci for phylogenetic
Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.q5gg0
- 摘要：
- range of ray-finned fishes (Class: Actinopterygii). We tested the primer pair using long-range PCR and Illumina sequencing in vitro on a mock community
Data from: Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model
- 负责人：
- DOI：
- doi:10.5061/dryad.8164
- 摘要：
- e a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR)
Data from: Multiplex preamplification PCR and microsatellite validation allows accurate single nucleotide polymorphism (SNP) genotyping of historical
- 负责人：
- Smith, Matt J.
- 关键词：
- fish scales Fisheries Management Population Genetics - Empirical historical DNA SNP preamplification
- DOI：
- doi:10.5061/dryad.8023
- 摘要：
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
- 负责人：
- DOI：
- doi:10.5061/dryad.6n7675r
- 摘要：
- can be ineffective due to the turtle’s wetland habitat, small size, and burrowing nature. Molecular methods, such as qPCR, provide the ability to overcome
Data from: Haemosporidian prevalence and parasitemia in the tufted titmouse (Baeolophus bicolor)
- 负责人：
- DOI：
- doi:10.5061/dryad.rd927
- 摘要：
- oscopy, polymerase chain reaction (PCR), and quantitative PCR (qPCR). Thirteen mitochondrial cytochrome b lineages of haemosporidian parasites were found including
Data from: Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats
- 负责人：
- DOI：
- doi:10.5061/dryad.nr2h5
- 摘要：
- ng polymerase chain reaction (PCR). The DNA viral load, genetic diversity, phylogeny and antibody titres against parvoviruses were investigated in the positive cats. Result
Data from: Multiple-strain infections of borrelia afzelii: a role for within-host interactions in the maintenance of antigenic diversity?
- 负责人：
- DOI：
- doi:10.5061/dryad.24k0s
- 摘要：
- reservoir hosts, the bank vole Myodes glareolus. We measured overall infection intensity with quantitative polymerase chain reaction (PCR) and r
Data from: A long PCR based approach for DNA enrichment prior to next-generation sequencing for systematic studies
- 负责人：
- DOI：
- doi:10.5061/dryad.kc75n
- 摘要：
- Premise of the study: We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing (NGS)
Data from: Fold or hold: experimental evolution in vitro
- 负责人：
- DOI：
- doi:10.5061/dryad.1nt11
- 摘要：
- quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures tha
