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Data from: “Direct PCR” optimization yields a rapid, cost-effective, non-destructive, and efficient method for obtaining DNA barcodes without DNA extraction
- 负责人：
- DOI：
- doi:10.5061/dryad.4q4kf
- 摘要：
- PCR method that skips DNA extraction (“direct PCR”) and that can be used for a broad range of invertebrate taxa. We demonstrate how direct PCR can be optimized
Data from: An examination of the accuracy of a sequential PCR and sequencing test used to detect the incursion of an invasive species: the cas
- 负责人：
- DOI：
- doi:10.5061/dryad.7652s
- 摘要：
- 1. Polymerase Chain Reaction (PCR) diagnostic tests are increasingly applied to the identification of wildlife. Yet rigorous verification is rare
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
- 负责人：
- DOI：
- doi:10.5061/dryad.591q3
- 摘要：
- . Here we describe the development and validation of a novel set of 91 nuclear markers for PCR-based target enrichment. Using microfluidic PCR
Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
- 负责人：
- DOI：
- doi:10.5061/dryad.fs728
- 摘要：
- can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem
Data from: Optimizing methods for PCR-based analysis of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.8947
- 摘要：
- alpine carabid beetles and lycosid spiders, we investigated how PCR annealing temperature affects prey DNA detection success and how post-PCR visualization
Data from: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol
- 负责人：
- Zarzoso-Lacoste, Diane
- 关键词：
- Invasive rats Diet Analysis PCR-based method Extraction protocol performances A260/A280 absorbance ratio Group-specific primer pairs
- DOI：
- doi:10.5061/dryad.8c134
- 摘要：
- itical step, as PCR inhibitors may be co-extracted. Another critical step consist in carefully select suitable group-specific primer sets that should
Data from: PCR-based isolation of multigene families: lessons from the avian MHC class IIB
- 负责人：
- DOI：
- doi:10.5061/dryad.1mn40
- 摘要：
- r a range of species are readily obtained by aligning conserved gene regions, and laborious gene isolation procedures can often be replaced by quicker PCR
Data from: Residual eDNA detection sensitivity assessed by quantitative real-time PCR in a river ecosystem
- 负责人：
- DOI：
- doi:10.5061/dryad.5rf92
- 摘要：
- eDNA detection sensitivity using quantitative real-time polymerase chain reaction (qRT-PCR), in a flowing, uncontrolled river after the eDNA source
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
- 负责人：
- Raquin Vincent
- DOI：
- doi:10.5061/dryad.pm71k02
- 摘要：
- ecially field-derived samples. Here, we developed a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay that discr
Data from: Blood-stage parasitaemia and age determine Plasmodium falciparum and P. vivax gametocytaemia in Papua New Guinea
- 负责人：
- DOI：
- doi:10.5061/dryad.550p3
- 摘要：
- and gametocytes by detection of pfs25 and pvs25 mRNA transcripts by reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by PCR was 18
