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Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods
- 负责人：
- DOI：
- doi:10.5061/dryad.2p51n
- 摘要：
- The quantification of the biological diversity in environmental samples using high-throughput DNA sequencing is hindered by the PCR bias caused
Data from: Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity
- 负责人：
- DOI：
- doi:10.5061/dryad.p3ks4
- 摘要：
- analyses, and it has been recommended as a core plant DNA barcode. Despite this popularity, the universality and specificity of PCR
Data from: Multiplex PCR targeting lineage specific SNPs ? A highly efficient and simple approach to block out predator sequences in molecular
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.44mn451
- 摘要：
- multiplex PCR, considerably reducing the necessary workload. Conclusions: Our multi-locus approach allows the generation of an unprecedented amount of prey
Data from: Calorespirometry reveals that goldfish prioritize aerobic metabolism over metabolic rate depression in all but near-anoxic environments
- 负责人：
- DOI：
- doi:10.5061/dryad.719bt
- 摘要：
- ng calorespirometry to test the hypothesis that MRD is employed at hypoxic PwO2s and initiated just below Pcrit, the PwO2 below which ?O2 is forced to progressively
Data from: Impact of microRNA expression in human atrial tissue in patients with atrial fibrillation undergoing cardiac surgery
- 负责人：
- DOI：
- doi:10.5061/dryad.8p1t6
- 摘要：
- microarrays and with Reverse transcriptase-polymerase chain reaction (RT-PCR). Fibrosis was examined using Masson trichrome staining. Results: miRNA
Data from: Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance
- 负责人：
- DOI：
- doi:10.5061/dryad.h373j
- 摘要：
- a set of synthetic oligonucleotides for use as species-specific positive PCR controls for several high-profile aquatic invasive species. The controls
Data from: Mitochondrial capture enriches mito-DNA 100 folds enabling PCR-free mitogenomics biodiversity analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.19nf6
- 摘要：
- Biodiversity analyses based on Next Generation Sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy
Data from: Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae
- 负责人：
- DOI：
- doi:10.5061/dryad.tf6r4
- 摘要：
- and other cell-based structures might accidentally attach as they are shed. This source of trace DNA can be sufficient for PCR amplification and sequencing
Data from: Improved detection of influenza A virus from blue-winged teals by sequencing directly from swab material
- 负责人：
- DOI：
- doi:10.5061/dryad.3h2n106
- 摘要：
- replication occurs mostly in the intestinal tract. Fecal, cloacal and/or tracheal swabs are typically collected and tested by real-time RT-PCR (rRT-PCR
Data from: Protocols for dry DNA storage and shipment at room temperature
- 负责人：
- DOI：
- doi:10.5061/dryad.6p2h0
- 摘要：
- and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica? DNAstable? plates, home-mad
