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Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment
Data from: Molecular prey identification in Central European piscivores
- 负责人：
- 关键词：
- Diet Analysis Piscivores Predator Prey Interactions Wildlife Management Diagnostic Multiplex PCR
- DOI：
- doi:10.5061/dryad.1vf74
- 摘要：
- using conventional morphological analysis of prey remains. Here, we present a two-step multiplex PCR system, comprised of six assays, allowing for rapid
Data from: A method for estimating population sex ratio for sage-grouse using noninvasive genetic samples
- 负责人：
- DOI：
- doi:10.5061/dryad.v0q5p
- 摘要：
- ng polymerase chain reaction (PCR) has become a common method for identifying sex from sources such as hair, feathers, or feces, and is a potential source for estimating sex ratio
Data from: Increasing the accuracy and precision of relative telomere length estimates by RT qPCR
- 负责人：
- DOI：
- doi:10.5061/dryad.7356h
- 摘要：
- , the costs of reproduction and disease. qPCR is a high throughput and cost effective tool to measure relative TL (rTL) that can be applied to newly-collected and archived
Data from: Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias
- 负责人：
- DOI：
- doi:10.5061/dryad.dn45c
- 摘要：
- estimates of diversity, metabarcoding markers ideally possess appropriate taxonomic coverage to avoid PCR-amplification bias, as well as sufficient
Data from: Whole transcriptome RNA-Seq analysis of breast cancer recurrence risk using formalin-fixed paraffin-embedded tumor tissue
- 负责人：
- DOI：
- doi:10.5061/dryad.q760h
- 摘要：
- RNA biomarkers discovered by RT-PCR-based gene expression profiling of archival formalin-fixed paraffin-embedded (FFPE) tissue form the basis
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
- 负责人：
- 关键词：
- Eurtytemora affinis
- DOI：
- doi:10.5061/dryad.rh7f4
- 摘要：
- , we developed a simple PCR-based method for obtaining sequence information from individual DNA strands using standard laboratory equipment. The method employs
Data from: 60 specific eDNA qPCR assays to detect invasive, threatened and exploited freshwater vertebrates and invertebrates in Eastern Canada
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.12jm63xtw
- 摘要：
- for which monitoring may be challenging. Species detection efforts using a combination of eDNA and qPCR have been highly successful and, as a result, thei
Data from: Multiplexed microsatellite markers for genetic studies of beech
- 负责人：
- DOI：
- doi:10.5061/dryad.pc8d30j2
- 摘要：
- climate change effects. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high throughput genotyping. Here we present the procedure use
Data from: Detection of airborne genetically modified maize pollen by real-time PCR
- 负责人：
- Folloni, Silvia
- DOI：
- doi:10.5061/dryad.82th6
- 摘要：
- PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated
