Provided are a biological product and a method of preparing same. The biological product is prepared by inoculating chicken embryo with a single-stranded RNA virus subjecting the recovered allantoic fluid to concentration by ultrafiltration, followed by purification to obtain a purified single-stranded RNA virus solution subjecting the allantoic fluid beneath the ultrafiltration membrane after the previous concentration to further concentration by ultrafiltration to obtain effective components beneath the ultrafiltration membrane and mixing the purified single-stranded RNA virus with the effective components beneath the ultrafiltration membrane, and then subjecting the mixture to sterilization by filtration to obtain said biological product. The biological product has the virus in high purity, and impurity proteins such as ovalbumin in the virus are few, thereby greatly reduces the side effects of the product on the human body. Also, the single-stranded RNA virus and the effective components beneath the ultrafiltration membrane have a synergistic effect against cancers, thus greatly increases the anti-tumor effect of the biological product. The method of preparation can effectively increase the purity of the virus and reduce the impurity proteins such as ovalbumin in the virus. The method is simple, can greatly decrease production cost, and is suitable for the needs of large-scale industrial production.Linvention concerne un produit biologique et son procédé de préparation. Le produit biologique est préparé par inoculation dun embryon de poulet avec un virus à ARN simple brin soumission du fluide allantoïque récupéré à une concentration par ultrafiltration, suivie par une purification pour obtenir une solution de virus à ARN simple brin purifié soumission du fluide allantoïque en dessous de la membrane dultrafiltration après la concentration précédente à une concentration supplémentaire par ultrafiltration pour obtenir des composants efficaces en dessous de la membr
