Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет пищевых производств" (RU)
发明人:
Колпакова Валентина Васильевна (RU),Фан Куинь Чам (RU),Чумикина Людмила Васильевна (RU)
申请号：
RU2012147113/10
公开号：
RU2012147113A
申请日:
2012.11.07
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. Biotechnological process for preparing a protein hydrolyzate from rice flour, rice flour comprising filling the flask and fill with tap water containing an amylolytic enzyme preparation in a concentration of 5.8 ... 6.2 amylase activity units per 1 g of raw material to the hydro-1: 7, processing for 75 ... 90 min at 65 ... 70 ° C and pH 5.8 ... 6.2 with stirring spilling the suspension to centrifuge tubes and centrifuged for 15 min at 6000 rev / min, the discharging centrifuge tubes and draining the supernatant solution, adding tap water to the residue, comprising Xylanase enzyme preparation at a concentration rate of 60 ... 70 units of activity per 1 g of raw material to the hydro-1: 6, the subsequent treatment for 2 hours at 65-70 ° C with stirring at an acidic pH of 4.8 with the simultaneous progress of the extraction process the proteins then re-centrifugation, the centrifuge tubes and discharging drain extract I in a separate flask, adding to the residue 0.01N HCl solution to hydronic 1: 9, followed by acid extraction at room temperature for 2 hours then repeated centrifugation to obtain ext rakta II, utilizirovanie residue extracts I and compound II, alkalization solution of 0.01N NaOH to pH 7.2, incubation 30 min at 4 ° C for the formation of colloidal aggregates centrifugation for 15 min at 5000 rev / min, washing with water precipitate, the partial hydrolysis with one endoprotease or a mixture of endo- and exoproteases in a concentration of 1.0 ... 1.4 U / g protein and supplemented with calcium citrate in a concentration of 0.02 ... 0.03 g / g of protein at 50 ... 60 ° C, the protein concentration of - 25 ... 30%, pH 5.5 ... 6.0 for 3 p.2. Biotechnological process for preparing a protein hydrolyzate from a rice1. Биотехнологический способ получения гидролизата белка из рисовой муки, включающий засыпку рисовой муки в колбу и заливку водопроводной водой, содержащей амилолитический ферментный препарат в концентрации 5,8…6,2 ед амилазной активности на 1 г с
