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[前沿资讯 ] 中国科学院深圳先进技术研究院发文揭示肠道共生菌联手噬菌体对抗南美白对虾弧菌病 进入全文
中国科学院深圳先进技术研究院
中国科学院深圳先进技术研究院马迎飞团队联合澳华集团，围绕南美白对虾副溶血弧菌病害，提出“共生菌-噬菌体协同防控”新策略。研究从健康对虾肠道分离12株共生菌，构建合成菌群Com12及精简版Com4，联合副溶血弧菌特异性噬菌体VP6phageC，在攻毒实验中将存活率由23%提升至69%。机制研究表明：菌群发育早期，致病弧菌易抢占生态位；引入关键共生菌VA3与噬菌体后，噬菌体迅速裂解VP6，VA3随即占位，形成连续防御；预防性干预显著优于延迟或治疗性处理。体外及模拟养殖实验证实，协同策略可在3天内清除水体弧菌，使肠道弧菌再降1个数量级，并提升菌群多样性，建立稳定微生态屏障。该成果为替代抗生素、实现绿色精准防控水产弧菌病害提供了可推广的微生态干预范式。
[前沿资讯 ] 北京大学研究通过人工智能辅助管道鉴定肠道微生物胆汁酸代谢酶 进入全文
科学网
北京大学姜长涛课题组报道了通过人工智能辅助管道鉴定肠道微生物胆汁酸代谢酶。相关论文发表在2025年8月7日出版的《细胞》杂志上。为了解决这个问题，研究组开发了一个人工智能（AI）辅助的工作流程，胆汁酸酶播音员单元工具（BEAUT），该工具预测了超过60万种候选BA代谢酶，并将其编译到人类通用微生物BA代谢酶（HGBME）数据库（https://beaut.bjmu.edu.cn）中。该研究团队鉴定了一系列未被鉴定的BA酶，包括单酸酰化BA水解酶（MABH）和3-乙酰DCA合成酶（ADS）。值得注意的是，ADS可以产生未报道的骨架BA， 3-acetoDCA，具有碳-碳键延伸。通过对3-acetoDCA的细菌存在和催化机制的测定，研究团队发现3-acetoDCA广泛分布于人群中，并调节肠道内微生物的相互作用。总之，他们的工作从酶的角度为微生物BAs和宿主之间的关系提供了另一种见解。
[学术文献 ] Unspecific peroxygenases and P450-derived peroxygenases as green biocatalysts driven by H2O2 for sustainable applications: A review 进入全文
International Journal of Biological Macromolecules
Unspecific peroxygenases (UPOs) are promising biocatalysts capable of catalysing selective oxygenation of organic substrates using hydrogen peroxide (H2O2) as the sole oxidant under mild conditions. Sharing a broad substrate range with cytochrome P450 monooxygenases, UPOs function without costly cofactors or auxiliary proteins, making them attractive for industrial biocatalysis. However, limited heterologous expression and poor H2O2 tolerance restrict their broader application. Recent advances in host selection and signal peptide optimisation have significantly improved UPOs production. Simultaneously, various in situ H2O2 generation systems—particularly photobiocatalytic strategies—have enhanced reaction efficiency by stabilising peroxide concentrations. Additionally, efforts to engineer P450s into peroxygenase-like enzymes aim to overcome the limitations of P450s in industrial applications and enhance their utility in organic synthesis. This review systematically summarizes the development of UPOs over the past decade, including technical bottlenecks and future application prospects. The review focuses on heterologous expression, in situ generation of H2O2, and the modification of P450s to boost peroxygenase activity, providing new ideas for addressing these challenges. In the future, UPOs and P450-derived peroxygenases are anticipated to play an increasingly significant role in green chemistry and organic synthesis.
[学术文献 ] Gram-per-litre-scale production of lutein by engineered Corynebacterium 进入全文
Nature Synthesis
Lutein is commonly used as a supplement for ocular health. However, commercial lutein is mainly extracted from marigold flowers, an inefficient process that includes secondary metabolites. Here, building on our previous research on lutein synthesis in engineered Escherichia coli, we report the development of a microbial platform for efficient lutein production using metabolically engineered Corynebacterium glutamicum. We engineered C. glutamicum to produce lycopene and introduced the lutein biosynthesis pathway. Then, we substantially enhanced lutein production by optimizing the haem pathway, engineering the P450 reductase and utilizing an optimal electron-channelling scaffold system to improve electron transfer reactions, addressing the rate-limiting steps in lutein synthesis. This approach achieved a substantial increase in lutein production, reaching a concentration of 1.78 g l−1, with a content of 19.51 mg per gram dry cell weight and a productivity of 32.88 mg l−1 h−1 in fed-batch fermentation. This approach allows gram-per-litre-scale microbial production of lutein.
[学术文献 ] A Unified Mechanism Reveals the Evolutionary Origin of Enoyl Isomerases 进入全文
ACS Catalysis
Enoyl isomerases (EIs) and dehydratases (DHs) are critical for diversifying polyketide natural products; however, the underlying mechanism remains controversial. Through a combination of 1H NMR, solvent isotope effect (SIE) analyses, and mutagenesis applied to two bifunctional dehydratase/enoyl isomerases (DH/EIs), a “His-solo” monofunctional EI, and an engineered “Asp-solo” monofunctional EI, we uncovered a unified catalytic mechanism, highlighting the pivotal role of Asp-H2O in tuning the ratio of DH and EI activities. This mechanism not only deciphers the catalytic basis of these enzymes but also provides a comprehensive framework for understanding their evolutionary trajectory from DHs to DH/EIs and ultimately to EIs.
[学术文献 ] Computational-assisted protein engineering to develop thermostable and highly active catalase for industrial and biocatalytic applications 进入全文
Bioresource Technology
Catalases are ideal biocatalysts for hydrogen peroxide removal in industrial wastewater and enzymatic processes, yet the practical application is hindered by poor thermostability. In this study, a highly active catalase was identified from Fictibacillus enclensis using a computer-assisted screening strategy. To overcome thermal instability, a computational design framework integrating ProteinMPNN-based sequence optimization with physics-based energy calculations was developed. The engineered variant, D78P/K201R/E384Y/T435A, exhibited a 1.9-fold increase in catalytic efficiency and a 4.9-fold extension of half-life at 40°C. Molecular dynamics simulations and structural analyses revealed that the mutations conferred enhanced global rigidity through stabilized hydrogen-bond networks. Moreover, the variant was employed for the treatment of industrial effluents containing hydrogen peroxide residues and the biocatalytic upgrading of glycerol. This study not only paved the way for industrial applications of catalase but also established a strategic framework for enhancing both stability and catalytic activity in enzyme engineering.
