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Data from: High-throughput microsatellite marker development in two sparid species and verification of their transferability in the family Sparidae
- 负责人：
- DOI：
- doi:10.5061/dryad.320kb06t
- 摘要：
- when enrichment was performed first before 454 sequencing. We designed primers for 302 unique fragments containing more than five repeat units and suitable flanking
Data from: Conserved genetic regions across angiosperms as tools to develop single copy nuclear markers in gymnosperms: an example using cycads
- 负责人：
- DOI：
- doi:10.5061/dryad.k2f0n
- 摘要：
- edictions were completed to assist in primer design. PCR was carried out in three rounds to detect paralogous loci. A total of 29 loci were successfully amplified
Data from: High-throughput genotyping of Anopheles mosquitoes using intact legs by Agena Biosciences iPLEX
- 负责人：
- DOI：
- doi:10.5061/dryad.nr716
- 摘要：
- Recent developments in genotyping technologies coupled with the growing desire to characterise genome variation in Anopheles populations opens
Data from: Next-generation sequencing for rodent barcoding: species identification from fresh, degraded and environmental samples
- 负责人：
- DOI：
- doi:10.5061/dryad.1j6v6
- 摘要：
- this fragment were designed. The efficacy of this new molecular tool was assessed on 946 samples including rodent tissues, feces, museum samples and feces/pellets
Data from: SNP discovery in European lobster (Homarus gammarus) using RAD sequencing
- 负责人：
- j.r.stevens@ex.ac.uk
- DOI：
- doi:10.5061/dryad.2pc6v
- 摘要：
- were quality filtered, ranked using F-statistics and the top 96 SNPs adequate for primer design were retained. SNP markers were developed with the aim
Data from: An empirical review: characteristics of plant microsatellite markers that confer higher levels of genetic variation
- 负责人：
- DOI：
- doi:10.5061/dryad.7gr39
- 摘要：
- During microsatellite marker development, researchers must choose from a pool of possible primer pairs to further test in their species of interes
Data from: Rapid identification of chloroplast haplotypes using High-Resolution Melting analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.s904k
- 摘要：
- analysis represents an untapped resource in phylogeographic analysis, and with appropriate primer design any polymorphic locus is potentially amenable
Data from: De novo transcriptome characterization and development of genomic tools for Scabiosa columbaria L. using next-gener
- 负责人：
- DOI：
- doi:10.5061/dryad.8160
- 摘要：
- for PCR primer design. We also identified 75,054 putative SNPs. This annotated sequence collection and the relative molecular markers represent a main genomic
Data from: DNA barcoding meets molecular scatology: short mtDNA sequences for standardized species assignment of carnivore noninvasive samples
- 负责人：
- DOI：
- doi:10.5061/dryad.75748
- 摘要：
- eliminated by the design of more specific primers. Overall, our results indicate that these segments hold good potential as standard markers for accurate
Data from: Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.55p06
- 摘要：
- ource to the beetle evolutionary research community for NPC marker development. We randomly chose 180 candidate loci from the database to design primers and succes
