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Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
- 负责人:
- DOI:
- doi:10.5061/dryad.179116t
- 摘要:
- oach assembled high-quality sequences, even if it did not recover all receptors in the genome. While some variation may be due to limitations of the study design
Data from: Sinonasal microbiome sampling: a comparison of techniques
- 负责人:
- DOI:
- doi:10.5061/dryad.cp8sr
- 摘要:
- : Cross-sectional study with paired design. Mucosal biopsy and swab samples were obtained intra-operatively from the ethmoid sinuses of 6 patients with CRS. Extracted DNA
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan
- 负责人:
- DOI:
- doi:10.5061/dryad.443t4m1q
- 摘要:
- adjustment relating to the design of the blocking oligonucleotide. It is robust, simple to implement, and allows the possibility of very large-scale analyses.
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
- 负责人:
- DOI:
- doi:10.5061/dryad.591q3
- 摘要:
- of these costs include reusable reagents (primers, barcodes, and custom sequencing primers) and taxon sampling could be increased by an order of magnitude
Data from: Rapid microsatellite marker development for African mahogany (Khaya senegalensis, Meliaceae) using next-gener
- 负责人:
- DOI:
- doi:10.5061/dryad.s03t592c
- 摘要:
- . The assembled sequences contained 1030 simple sequence repeats, with primers designed for 522 microsatellite loci. Twenty one microsatellite loci were tes
Data from: De novo transcriptome assemblies of four accessions of the metal hyperaccumulator plant Noccaea caerulescens
- 负责人:
- DOI:
- doi:10.5061/dryad.380n3
- 摘要:
- , and for primer design.
Data from: Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent
- 负责人:
- DOI:
- doi:10.5061/dryad.36775
- 摘要:
- s of interspecific hybridization. We use transcriptome data of a single Triturus species and design 96 primer pairs that amplify c. 180 bp fragments positioned in 3-pri
Data from: Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia)
- 负责人:
- DOI:
- doi:10.5061/dryad.62cr1q22
- 摘要:
- e brown bat, Myotis lucifugus to create consensus sequences which were used to design candidate primer sets. Primer sets were then tested for amplification
Data from: Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
- 负责人:
- DOI:
- doi:10.5061/dryad.rc47tt4
- 摘要:
- s of nucleotide substitution rates for 2,389 unigenes indicated that 39 genes were under strong positive selection. The primers of 6,911 microsatellites were designed, and 40
Data from: eSnail: a transcriptome-based molecular resource of the central nervous system for terrestrial gastropods
- 负责人:
- DOI:
- doi:10.5061/dryad.41r85
- 摘要:
- single nucleotide variants, which can be used for the design of potentially thousands of unique primers for functional screening. An online ‘eSnail’ database