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Data from: Scrimer: designing primers from transcriptome data
- 负责人：
- DOI：
- doi:10.5061/dryad.2p4t3
- 摘要：
- e is no publicly available software for the automated design of suitable PCR and genotyping primers from next-generation sequence data. Here we present a pipeline
Data from: PrimerMiner: an R package for development and in silico validation of DNA metabarcoding primers
- 负责人：
- DOI：
- doi:10.5061/dryad.4c3g9
- 摘要：
- ated with PrimerMiner can be used for manual primer design, or processed with bioinformatic tools for primer development.
Data from: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels
- 负责人：
- DOI：
- doi:10.5061/dryad.4m0v8474
- 摘要：
- sequencing, replication, and oligonucleotide synthesis errors do not cause tags to be unrecoverable or confused. However, many design approaches only pr
Data from: Efficient detection of novel nuclear markers for Brassicaceae by transcriptome sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.63j2j
- 摘要：
- The lack of DNA sequence information for most non-model organisms impairs the design of primers that are universally applicable for the study
Data from: Slippage of degenerate primers can cause variation in amplicon length
- 负责人：
- DOI：
- doi:10.5061/dryad.nk81st2
- 摘要：
- ould be taken to reduce slippage by improving protocols for primer design. For example, the flanking region adjacent to the 3? end of the primer
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- for genes with low expression levels, but not for a highly expressed gene. By contrast, significant knockdown was detected in all cases with primer
Data from: A broadly applicable COI primer pair and an efficient single?tube amplicon library preparation protocol for metabarcoding
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.5jq5t36
- 摘要：
- , this variability also makes it difficult to design truly universal primers. Here, we present the forward primer “Sauron-S878”, specifically designed to facilitate
Data from: Non-specific amplification compromises environmental DNA metabarcoding with COI
- 负责人：
- DOI：
- doi:10.5061/dryad.b8f6s44
- 摘要：
- sment. However, choice of genetic marker and primer set is a critical consideration when designing experiments, especially so when developing community standards
Data from: A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application
- 负责人：
- 关键词：
- second generation sequencing DNA barcoding mini-barcode mitochondrial marker trophic interactions food web
- DOI：
- doi:10.5061/dryad.6gd51
- 摘要：
- orly conserved across some taxonomic groups. Results: We first design a new PCR primer within the highly variable mitochondrial COI region, the “mlCOIintF” primer
Data from: Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n8v4v6d
- 摘要：
- instruments at reduced cost with increased flexibility. A simple web page to design fusion primers compatible with iTru primers is available at: http://baddna
