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Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community
- 负责人：
- DOI：
- doi:10.5061/dryad.tn6vn
- 摘要：
- dollar, many researchers are exploring HTS for microbial community ecology. Many elements of experimental design can drastically affect the final obser
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- of designing species-specific primers can result in significant expenditure of time and money. During the in silico stage of primer development, primer
Data from: Adapterama III: quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
- 负责人：
- DOI：
- doi:10.5061/dryad.tr87dh0
- 摘要：
- robust approach for the construction of dual-digest RADseq libraries that relies on adapters and primers designed in Adapterama I. Major features
Data from: Microsatellite markers from the Ion Torrent: a multi-species contrast to 454 shotgun sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.vv82q
- 摘要：
- in the quantity of returned sequence data, unique microsatellite data and primer design opportunities, mostly consistent with the differences in read length
Data from: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical
- 负责人：
- DOI：
- doi:10.5061/dryad.54v2q
- 摘要：
- We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned
Data from: A pragmatic approach to the analysis of diets of generalist predators: the use of next-generation sequencing with no blocking probes
- 负责人：
- DOI：
- doi:10.5061/dryad.qv633
- 摘要：
- the need to design and test numerous blocking primers, thus making analysis of complex community food webs a viable proposition. We applied this appr
Data from: Effects of sampling effort on biodiversity patterns estimated from environmental DNA metabarcoding surveys
- 负责人：
- DOI：
- doi:10.5061/dryad.40782nd
- 摘要：
- on results of this study, we make several recommendations for port eDNA sampling design and suggest several areas for future research.
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
- 负责人：
- DOI：
- doi:10.5061/dryad.8271f
- 摘要：
- , and composition. The aim of this study was to examine how tissue storage prior to DNA extraction, primer design, and various quality-control approaches commonly use
Campylomormyrus compressirostris (Mormyridae, Teleostei)
- 负责人：
- DOI：
- doi:10.5061/dryad.ns135
- 摘要：
- -electric species. Additionally we located 1671 SSR containing regions with their flanking sites and designed the relative primers. The present study
Data from: Characterization of an intergenic polymorphic site (pp-hC1A_5) in Wolbachia pipientis (wPip)
- 负责人：
- DOI：
- doi:10.5061/dryad.8289
- 摘要：
- complex. Here we present a method useful for genotyping within the w Pip group. Primers were designed using a Tandem Repeat Finder program to amplify
