Disclosed herein are a growth medium for cultivatingGanoderma lucidum, preparation method and uses thereof. Specifically, the growth medium has 90-110 parts by weight of aGanoderma lucidumwaste, 3-10 parts by weight of a tapioca starch, 1-5 parts by weight of a glucose, and 1 parts by weight of a soy flour； wherein the growth medium is characterized in having a water content of about 60-80% and not having sawdust and/or baboo dust therein. Moreover, the growth medium of present disclosure is for cultivatingGanoderma lucidumused in the sustainable agriculture to reduce the waste, and to increase the content of triterpenoids in Ganoderma lucidum.在此揭露一種利用廢棄靈芝渣所製成的靈芝培養基、製備靈芝培養基的方法和應用。具體而言，所述靈芝培養基含有90-110重量份的靈芝渣、3-10重量份的樹薯粉、1-5重量份的葡萄糖和1重量份的黃豆粉，其中該靈芝培養基的水含量為60-80%，且該靈芝培養基不包含木屑和/或竹粉。本揭示內容所提出的靈芝培養基，除了可有效再利用靈芝渣，減少廢棄物外，還可由所培育的靈芝獲取更多活性成分，達到循環利用並生產的目的，。
