A kind of anti-avian influenza virus medicament, it contains ingredient acquired when making to be separated under the happy extract what specified conditions of a fragrant plant by HPLC, and the happy extract system of the fragrant plant obtains by using the extractant using at least one of water and hydrophilic organic solvent as principal component from the happy extraction of fragrant plant. The specified conditions include: using internal diameter 20mm and the ODS tubing string of length 250mm； The happy extract of what fragrant plant put into tubing string Hou to when 15 minutes, use water-methanol mixture that methanol concentration is 20% as mobile phase, Hou what its investment when Hou 15 ~ 30 minutes, use water-methanol mixture that methanol concentration is 40% as movement phase； And the flow velocity of mobile phase is 10mL/ minutes. Alternatively, the specified conditions further include: when the happy extract of what fragrant plant is put into Hou 30 ~ 45 minutes, using water-methanol mixture that methanol concentration is 60% as moving phase. The anti-avian influenza virus medicament contains the happy extract of the what fragrant plant and puts into Hou to the ingredient for eluting out from tubing string when 20 minutes or when what its investment Hou 20 ~ 35 minutes as effective component.一種抗禽流感病毒藥劑，其含有使一芭樂萃取物於特定條件下藉由HPLC進行分離時所得到之成分，該芭樂萃取物係藉由使用以水及親水性有機溶劑中至少一種作為主成分之萃取溶劑從芭樂萃取而得。該特定條件包含：使用內徑20mm且長度250mm之ODS管柱；於芭樂萃取物投入管柱後至15分鐘之期間，使用甲醇濃度為20%之水-甲醇混合物作為移動相，之後於其投入後15~30分鐘之期間，使用甲醇濃度為40%之水-甲醇混合物作為移動相；且移動相之流速為10mL/分鐘。或者，該特定條件進一步包含：於芭樂萃取物投入後30~45分鐘之期間，使用甲醇濃度為60%之水-甲醇混合物作為移動相。該抗禽流感病毒藥劑含有於該芭樂萃取物投入後至20分鐘之期間或於其投入後20~35分鐘之期間從管柱洗提出來之成分作為有效成分。
