The mushroom cultivation method of the present invention comprises the steps of: preparing a mushroom cultivation raw material; sterilizing the mushroom cultivation raw material; and placing the sterilized mushroom cultivation raw material in a sterile environment to cool; sterilizing and cooling the mushroom cultivation raw material after implantation; filling the mushroom cultivation raw material into a container having a columnar cylinder; after the mushroom is grown, the container is removed to expose the mushroom cultivation material having a columnar surface. Thereby, a mushroom cultivation method capable of shortening the sterilization time, saving energy, not generating disposable plastic waste, and increasing the productivity can be provided.本發明之香菇栽培方法包含以下步驟:製備香菇栽培原料;將該香菇栽培原料進行滅菌;將滅菌後的該香菇栽培原料置於無菌環境冷卻降溫;於滅菌且降溫後的該香菇栽培原料植入菌體;將已植入菌體的該香菇栽培原料裝填至具有柱狀筒體之容器;待菌絲體成長後移除容器,露出形成有柱狀表面的該香菇栽培原料。藉此提供一種能縮短滅菌時間、節省能源、不產生一次性塑膠廢料,而且能使產能增加的香菇栽培方法。