PURPOSE: A medium material composition for cultivating mushroom using actinidia chinensis, and a mushroom cultivation method using thereof are provided to use fermented fruits for cultivating the mushroom. CONSTITUTION: A mushroom cultivation method using a medium material composition comprises the following steps: supplying water to the medium material composition to obtain a medium composition having the water content of 40-70wt%; sterilizing the medium sterilizing the medium composition at 100-130 deg C for 60-150 minutes, and cooling; injecting pleurotus ostreatus spawns into the medium composition, and cultivating at 20-25 deg C; and inducing the generation of fruit bodies from the spawns for growing the mushrooms.본 발명은 침엽수인 일종인 삼나무의 톱밥과 참다래 과실·목재 혼합물의 발효물을 이용한 표고버섯, 느타리버섯 또는 노루궁뎅이버섯 재배용 배지 원료 조성물 및 이를 이용한 버섯의 재배 방법을 개시한다.