PROBLEM TO BE SOLVED: To provide a liquid koji that has effect for saltiness enhancement, and a seasoning liquid in which saltiness is strong and overall palatability is excellent even under non-salt or low salt condition using the liquid koji.SOLUTION: Aspergillus is cultured in a liquid culture medium including a specified quantity of glycine or other amino acids to produce liquid koji. The obtained liquid koji is added to a food material by non-salt or under the presence of low salt, then performed by hydrolysis at from 42°C to 57°C, thereby an objective seasoning liquid is obtained.