表面プラズモン励起増強蛍光分光法を利用して蛍光量を測定する定量分析方法ならびにそれに用いられる定量分析用キットおよびアナライト解離抑制剤
- 专利权人:
- 发明人:
- 申请号:
- JP20110191571
- 公开号:
- JP5754309(B2)
- 申请日:
- 2011.09.02
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要:
PROBLEM TO BE SOLVED: To provide a quantitative analysis method capable of inhibiting dissociation between a ligand and an analyte and performing a highly sensitive and precise detection with suppressed variation in obtained signals in the quantitative analysis method for measuring the fluorescence amount using surface plasmon field-enhanced fluorescence spectroscopy, a quantitative analysis kit used therefor, and an analyte dissociation inhibitor.
SOLUTION: A quantitative analysis method according to the present invention includes a specific sensor chip, nanostructured particles, and specific third ligands. The quantitative analysis method for an analyte by surface plasmon field-enhanced fluorescence spectroscopy uses a nanostructure formed by fixing a specific second ligand and the third ligand on the nanostructured particle. The quantitative analysis method includes performing predetermined processes.
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- 来源网站:
- 中国工程科技知识中心