Disclosed is a Spirulina extract solution for liver regeneration, and pharmaceutical composition and use thereof. The Spirulina extract solution is produced by a low-temperature disintegrating manufacture, and the solution contains a Spirulina bioactive material selected from the group consisting of allophycocyanin, calcium spirulan, C-phycocyanin and the mixture thereof.本發明揭露一種提升一肝病變個體的肝臟功能之螺旋藻提取液,該螺旋藻提取液係將含有藍藻之溶液經低溫崩解的萃取方法後所製成,且該螺旋藻提取液包括一生物活性物質,該生物活性物質係選自由異藻藍蛋白、含硫多醣體、C-藻藍蛋白及其混合物所組成之群組。