A composition is provided, which comprises aCistanchetubulosaextract as a first component and ubiquinone (Q10) and/or ubiquinol (QH) as a second component at a weight ratio of first component: second component = 0.67: 1 to 10: 1. The composition is an anti-fatigue pharmaceutical composition, an anti-fatigue food composition, an anti-fatigue cosmetic composition, an anti-fatigue care product composition, or an anti-fatigue feed composition, wherein the pharmaceutical composition is for treating or preventing chronic fatigue syndrome, and the food composition, the cosmetic composition, the care product composition, and the feed composition is for at least one of enhancing endurance performance, enhancing athletic ability, enhancing physical strength, recovering physical strength, promoting fatigue elimination, preventing myocardial weakness, helping to ameliorate myocardial weakness and helping to improve cardiac function.一種組合物，其係包含作為第一組分之管花肉蓯蓉（Cistanchetubulosa）萃取物以及作為第二組分之氧化型輔酵素（Q10）及／或還原型輔酵素（QH），其中該第一組分與該第二組分之重量比為第一組分：第二組分 = 0.67：1至10：1。該組合物係一抗疲勞之醫藥組合物、食品組合物、化妝品組合物、保養品組合物、或飼料組合物，且該醫藥組合物係可用於治療或預防慢性疲勞症候群，該食品組合物、化妝品組合物、保養品組合物、及飼料組合物係可用於以下至少一者：提高耐力表現、提高運動能力、增強體力、恢復體力、促進疲勞消除、預防心肌無力、幫助心臟無力改善、及幫助心臟機能改善。
