A composition and its use in anti-fatigue are provided, wherein the composition comprises a first component being a Cistanche tubulosa extract and a second component being ubiquinone (Q10) and/or ubiquinol (QH), and the weight ratio of the first component to the second component ranges from 0.67:1 to 10:1. The composition is used for anti-fatigue and is a pharmaceutical composition, a food composition, a cosmetic composition, a care product composition, or a feed composition. The pharmaceutical composition is used for treating or preventing chronic fatigue syndrome, and the food composition, the cosmetic composition, the care product composition and the feed composition are used for at least one of increasing endurance performance, increasing athletic ability, increasing physical strength, recovering physical strength, promoting fatigue elimination, preventing myocardial weakness, helping ameliorate myocardial weakness and helping improve cardiac function.一種抗疲勞物品，其係包含一第一部分以及一第二部分，其中，該第一部份係一殼體且具一容置空間，該第二部分係一充填物且存在於該容置空間中，且其中，該充填物係包含一抗疲勞組合物，該抗疲勞組合物係包含作為第一組分之管花肉蓯蓉(Cistanche tubulosa)萃取物，以及作為第二組分之氧化型輔酵素(Q10)及/或還原型輔酵素(QH)，其中該第一組分與該第二組分之重量比為第一組分：第二組分=0.67：1至10：1。此一抗疲勞物品係可用於以下之至少一者：治療慢性疲勞症候群、預防慢性疲勞症候群、提高耐力表現、提高運動能力、增強體力、恢復體力、促進疲勞消除、預防心肌無力、幫助心臟無力改善、及幫助心臟機能改善。
