Droguett, Gustavo,Frendewey, David,Zambrowicz, Brian,Voronina, Vera,Macdonald, Lynn,Prissette, Marine,Lai, Ka-Man Venus,Badithe, Ashok,Murphy, Andrew J.
申请号：
AU20170268458
公开号：
AU2017268458(A1)
申请日:
2017.05.19
申请国别(地区):
澳大利亚
年份:
2018
代理人:
摘要：
Methods and compositions are provided for making non-human animals with reduced tolerance of a foreign antigen of interest and making antigen-binding proteins against that foreign antigen of interest using such animals. The methods and compositions employ CRISPR/Cas9 systems using multiple guide RNAs to reduce or eliminate expression of a self-antigen homologous to or sharing an epitope of interest with the foreign antigen of interest or to reduce or eliminate expression of an epitope on the self-antigen that is shared with the foreign antigen of interest.
