Vera Voronina,Lynn Macdonald,Marine Prissette,Ka-Man Venus Lai,Ashok Badithe,Andrew J. Murphy,Gustavo Droguett,David Frendewey,Brian Zambrowicz
申请号：
US15600466
公开号：
US20170332610A1
申请日:
2017.05.19
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Methods and compositions are provided for making non-human animals with reduced tolerance of a foreign antigen of interest and making antigen-binding proteins against that foreign antigen of interest using such animals. The methods and compositions employ CRISPR/Cas9 systems using multiple guide RNAs to reduce or eliminate expression of a self-antigen homologous to or sharing an epitope of interest with the foreign antigen of interest or to reduce or eliminate expression of an epitope on the self-antigen that is shared with the foreign antigen of interest.
