VORONINA, VERA,MACDONALD, LYNN,PRISSETTE, MARINE,LAI, KA-MAN VENUS,BADITHE, ASHOK,MURPHY, ANDREW J.,DROGUETT, GUSTAVO,FRENDEWEY, DAVID,ZAMBROWICZ, BRIAN
申请号：
CA3022997
公开号：
CA3022997A1
申请日:
2017.05.19
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
Methods and compositions are provided for making non-human animals with reduced tolerance of a foreign antigen of interest and making antigen-binding proteins against that foreign antigen of interest using such animals. The methods and compositions employ CRISPR/Cas9 systems using multiple guide RNAs to reduce or eliminate expression of a self-antigen homologous to or sharing an epitope of interest with the foreign antigen of interest or to reduce or eliminate expression of an epitope on the self-antigen that is shared with the foreign antigen of interest.
