PURPOSE: A producing method of canned food using acipenser sinensis is provided to offer the chewy texture and the savory taste of the acipenser sinensis. CONSTITUTION: A producing method of canned food using acipenser sinensis comprises the following steps: defrosting acipenser sinensis in water for 2~6hours, removing cephalic segments and intestines, and washing the obtained fish meat dipping the fish meat in a marinade composition containing green tea and herbs for 12hours, and steaming cooling the fish meat for 12~24hours, cutting the fish meat into the size for an empty can, and washing the empty can inserting the fish meat, and adding canola oil, green tea cream sauce, red pepper sauce, or teriyaki sauce before sealing the can and sterilizing the sealed can at 115deg C and cooling.본 발명은 통조림 어육의 초기 병원성 미생물 생육억제와 조직감 향상을 위하여 마리네이드 조성물에 원료 해동, 두절, 내장제거 및 세척과 같이 전처리한 철갑상어 육을 실온에서 12시간 침지하여서 철갑상어 통조림을 제조하는 방법을 제공하기 위한 것이다.