The present invention relates to a method of manufacturing an animal feed additive by using artemisia capillaris, huttuynia cordata, and perilla frutescens. The method includes: a suspending step of suspending 1-2 g of leaf mold and 45-55 ml of water in 10 ml of physiological saline, and then, removing floating matters in the upper part a diluting step of making a diluted solution by diluting supernatant liquid, obtained from the suspended mixture, in physiological saline at ten times the original state, and then, repeatedly diluting the diluted solution 2-4 times at ten times the original state a seed culturing step of inserting 1-2 g of artemisia capillaris powder, 0.1-0.2 g of mulberry leaf powder, and 250-350 ml of distilled water into a bubble generating device based on 1 ml of the final diluted solution, and then, culturing the mixture at 25-35°C for one week to separate microorganism seeds by succeeding the seeds three or four times a primary culturing step of mixing 500-700 cc of the extracted microorganism seeds, 250-350 kg of mulberry leaves, and 100-200 kg of mulberry tree branch powder with 1 t of potable underground water, and then, culturing the mixture at room temperature for 2-4 months while supplying oxygen by operating a bubble maker a secondary culturing step of mixing the cultured liquid with 250-350 kg of artemisia, 150-250 kg of huttuynia cordata, and 50-150 kg of perilla frutescens, and then, continuing to culture the mixture at a room temperature for 1-2 months and an aging step of aging the cultured liquid for 1-2 months.COPYRIGHT KIPO 2018본 발명은 인진쑥, 어성초, 및 자소엽을 이용한 동물 사료 첨가제의 제조방법에 관한 것으로서, 부엽토 1~2g과 물 45~55㎖를 생리식염수 10㎖에 현탁한 후 상층부에 존재하는 부유물을 제거하는 현탁단계 상기 현탁한 혼합물에서 상층액을 취해서 생리식염수로 10배 희석하여 희석액을 제조하고 상기 희석액을 다시 2~4회 반복하여 10배 희석하는 희석단계 상기 희석단계를 거친 최종 희석액 1㎖를 기준으로 인진쑥분말 1~2g, 뽕잎 분말 0.1~0.2g, 증류수 250~350㎖를 기포 발생장치에 투입하고 이를 25~35℃에서 일주일간 배양하여 미생물 종균이 나타나면 3~4회 계대 배양하여 분리하는 종균배양단계 식용가능한 지하수 1t을 기준으로 상기 추출한 미생물 종균 500~700cc와 뽕잎 250~350kg, 뽕나무가지
