A method for the extraction and purification of conjugated triennial linoleic acid (CLN).The method comprises the following steps: (a) the extraction step: placing the Momordica charantia seed sample in the extraction container,The crude extract of balsam pear seed oil was extracted by supercritical fluid.(b) esterification step: esterification reaction takes place between the crude extract of balsam pear seed oil obtained in (a) and esterifier to form esterified balsam pear seed oil；And (c) purification step: to simulate a moving bed,The esterified unsaturated fatty acid is separated from the esterified bitter melon seed oil obtained in (b) above.The balsam pear seed oil with more than 50% by weight of conjugated triennial linoleic acid (CLN) was obtained.一種共軛三烯次亞麻油酸 (CLN)之萃取純化方法，其係包括：(a)萃取步驟：將苦瓜籽樣品置於萃取容器中，利用超臨界流體萃取出苦瓜籽油粗萃物；(b) 酯化步驟：將上述(a)所得到之該苦瓜籽油粗萃物與酯化劑發生酯化反應而形成酯化苦瓜籽油；以及(c) 純化步驟：以模擬移動床，從上述(b)所得到之該酯化苦瓜籽油中分離出酯化不飽和脂肪酸，而獲得具有共軛三烯次亞麻油酸 (CLN)之總含量超過50重量%的苦瓜籽油。
