The present invention provides a use of bitter gourd seed oil for the preparation of an antibody fat forming preparation by administering an effective amount of bitter melon seed oil to a desired individual； wherein the individual carries a specific obesity gene variation. Bitter melon seed oil not only increases the content of high-density cholesterol, increases calorie expenditure, increases fatty acid oxidation, inhibits body fat formation, and does not cause side effects of liver damage, sympathetic nerves, and parasympathetic over-activation after taking it. Developed a safe and effective body fat-lowering health food.本發明提供一種苦瓜籽油用於製備抗體脂肪形成製劑之用途，其係施予一有效劑量之苦瓜籽油至所需個體；其中該個體帶有特定肥胖基因變異。苦瓜籽油不但能夠增加高密度膽固醇之含量、增加熱量支出，增加脂肪酸氧化、抑制體脂肪形成，使用者服用後亦不會引發肝損傷、交感神經、及副交感神經過於活化之副作用，藉此能夠開發出安全有效的降體脂保健食品。
