A method for extracting active ingredients from bitter gourd includes an extraction step of extracting a bitter gourd composition using an ultrasonic extraction device to obtain a bitter gourd extract. The ultrasonic extraction device includes an ultrasonic generator and a working rod. The ultrasonic generator includes an oscillator that can generate ultrasonic waves with a frequency of 20 or 28 kHz. Wherein, when the ultrasonic frequency generated by the oscillator of the ultrasonic generator is 20 kHz, the method further includes a hydrolysis step of hydrolyzing the bitter gourd extract. The method of the present invention can effectively extract active ingredients (such as peptides and momordica glycosides) from bitter gourd, and can also reduce the time required for extracting active ingredients from bitter gourd.一種從苦瓜中提取出活性成分的方法,包含一利用一超音波萃取裝置對一苦瓜組成物進行萃取而得到一苦瓜萃取物的萃取步驟。該超音波萃取裝置包含一超音波產生器及一工作桿。該超音波產生器包括一震盪子,該震盪子能產生頻率為20或28 kHz的超音波。其中,當該超音波產生器的震盪子所產生的超音波頻率為20 kHz時,該方法還包含一對該苦瓜萃取物進行水解的水解步驟。本發明方法除了能有效從苦瓜中提取出活性成分(例如胜肽及苦瓜苷)外,還能減少從苦瓜中提取出活性成分所需要的時間。