methods for producing a phytase variant, for enhancing the nutritional value of an animal feed, for treating vegetable protein, for synthesizing a fermentation product, and for producing ethanol, phytase variant, polynucleotide, nucleic acid construct, vector of recombinant expression, recombinant host cell, transgenic plant or plant part, composition, process for reducing phytase levels in animal manure, and use of phytase variant or composition in animal feed
LARS KOBBEROEE SKOV,LEONARDO DE MARIA,MICHAEL SKJOET
申请号：
BR112012023822
公开号：
BR112012023822A2
申请日:
2011.03.25
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
methods for producing a phytase variant, for enhancing the nutritional value of an animal feed, for treating vegetable protein, for synthesizing a fermentation product, and for producing ethanol, phytase variant, polynucleotide, nucleic acid construct, vector of recombinant expression, recombinant host cell, transgenic plant or part by plant, composition, process for reducing phytase levels in animal manure, and use of the phytase variant or composition in animal feed the present invention relates to a method for produce phytase variants which have at least 70% identity with a buttiauxella-derived phytase, and comprise at least one additional disulfide bond compared to this phytase. These phytase variants have modified, preferably improved, properties such as thermostability, temperature profile, ph profile, specific activity, animal feed performance, lower protease sensitivity and / or a modified glycosylation pattern. The invention also relates to the variants produced, encoding these phytases, methods of their production, as well as their use, for example, in animal feed and in animal feed additives.métodos para produzir um variante de fitase, para melhorar o valor nutricional de uma ração animal, para o tratamento de proteínas vegetais, para sintetizar um produto de fermentação, e para produzir etanol, variante de fitase, polinucleotídeo, construto de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, planta transgênica ou parte a planta, composição, processo para reduzir os níveis de fitase no esterco animal, e, uso do variante de fitase ou da composição na ração animal a presente invenção refere-se a um método para produzir variantes de fitase que apresentam pelo menos 70% de identidade com uma fitase derivada de buttiauxella, e compreendem pelo menos uma ligação dissulfeto adicional comparado a esta fitase. estes variantes de fitase modificaram, preferivelmente melhoraram, propriedades tais como termoestabilidade, perfil de tempe
