The present invention relates to a conjugate comprising a physiologically active substance, a linker, and a substance capable of increasing the in vivo half-life of the physiologically active substance, a method for producing the conjugate, and a preparation thereof. [Selection diagram] Fig. 1本発明は、生理活性物質、リンカー、及び生理活性物質の生体内半減期を増加させうる物質を含む、結合体、その製造方法、及びその製剤に関する。【選択図】図1