The Institute of Biophysics Chinese Academy of Sciences
发明人:
申请号：
EP16877539.3
公开号：
EP3395329A4
申请日:
2016.11.22
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
A preparation method of adiposomes, and use thereof. Particularly, provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising the following steps: a1) vortexing phospholipid and neutral lipids in a buffer to enable the reaction between both, then performing centrifugation and collecting an upper liquid phase； a2) performing purification on the upper liquid phase obtained in the step a1) for twice or more, wherein the process of each purification is: uniformly mixing the upper liquid phase with the buffer, then layering the mixture, and collecting an upper liquid phase； and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in which the adiposomes are contained. For the adiposomes prepared by the method, one or more resident proteins and/or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins； and they all play important roles in preparing drugs and/or drug carriers.
