ZHAO, LISHAN,LI, WENZONG,WICHMANN, GALE,KHANKHOJE, ADITI,GARCIA DE GONZALO, CHANTAL,MAHATDEJKUL-MEADOWS, TINA,JACKSON, SHAINA,LEAVELL, MICHAEL,PLATT, DARREN
申请号：
CA3031162
公开号：
CA3031162A1
申请日:
2017.08.11
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
