[PROBLEMS] To provide a seasoning production method, seasoning, umami enhancer, which can be added to foods and drinks, has a good flavor, and can impart umami, salty taste, sweet taste, pre-taste, aftertaste, meat flavor, etc. Provision of a salty taste enhancer, a meat flavor enhancer, and a food or drink containing the seasoning. A glutamic acid-rich yeast extract having a glutamic acid content of 10% by mass or more is contained in an amount of 3% by mass to 24% by mass, and a glutathione-rich yeast extract having a glutathione content of 5% by mass or more is contained in an amount of 5% by mass to 25% by mass. It is a manufacturing method of the seasoning characterized by heating a mixture.【課題】飲食品へ添加できる、良好な風味を持ち、なおかつ、旨味、塩味、甘味、先味、後味、ミートフレーバーなどを付与することができる調味料の製造方法、調味料、旨味増強剤、塩味増強剤、ミートフレーバー増強剤、及び該調味料を含む飲食品の提供。【解決手段】グルタミン酸含量が10質量%以上であるグルタミン酸高含有酵母エキス3質量%~24質量%、及びグルタチオン含量が5質量%以上であるグルタチオン高含有酵母エキス5質量%~25質量%を含む混合物を加熱することを特徴とする調味料の製造方法である。