A manufacturing method of coffee bean with cordyceps militaris content includes following steps: (a) pure culturing the cordyceps militaris with a liquid medium (b) sterilizing the coffee bean under a high temperature and high pressure environment, and cooling the coffee bean by air drying (c) adding a nutrient fluid having 30g/L soluble starch and 10g/L soybean protein, adding a liquid medium, and placing the coffee bean in an incubator under a culturing temperature to inoculate the coffee bean (d) culture accelerating, wherein the incubator is configured under a culture acceleration temperature lower than a culturing temperature to carry out an illuminating culturing, such that the hypha and carpophore of cordyceps militaris grow from the coffee bean (e) maturing the hypha and carpophore by configuring the incubator to the culturing temperature to carry out the illuminating culturing and (f) drying to acquire the coffee bean with cordyceps militaris content.本發明提供一種具有蛹蟲草的咖啡豆之製備方法,其包含步驟:(a)純化:係將蛹蟲草藉由液態培養基純化培養;(b)滅菌:將咖啡豆以高溫高壓方式滅菌,並風乾冷卻;(c)接種:將咖啡豆添加一培養液,培養液包含30g/L的可溶澱粉及10g/L的大豆蛋白,再加入液態培養基,放置於一培養溫度之一培養箱進行無光培養;(d)加速培養:接著把培養箱設定於一加速培養溫度進行光照培養,使咖啡豆長出蛹蟲草之菌絲及子實體,加速培養溫度低於培養溫度;(e)成熟培養:將培養箱升溫至培養溫度,並且進行光照培養;以及(f)乾燥:最後進行乾燥得到具有蛹蟲草之咖啡豆。S1‧‧‧純化S2‧‧‧滅菌S3‧‧‧接種S4‧‧‧加速培養S5‧‧‧成熟培養S6‧‧‧乾燥
