An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 3' and 5' ends) at the ETIP genomic location. One element in the invention is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, TALs, CRISPRs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.本文描述一種工程基因轉殖整合平台(ETIP)，其可被隨機地或在植物基因組之標的位置插入，用以協助快速選擇及偵測於ETIP基因位置被完全標定(3'及5'端二者)之GOI。本發明要素之一係在ETIP中引入特異性雙鏈斷裂。某些具體實施例中描述一種使用鋅指核酸酶(結合位之ETIP，但可能使用其他標定技術，如大範圍核酸酶(meganucleases)、TALs、CRISPRs或白胺酸拉鏈。本文亦描述用以產生基因轉殖植物的組成物和方法，其中，提供者或有效負載(payload)之DNA表現一或多種外源核酸序列之產物(例如蛋白質或RNA)，該產物被穩定結合至一植物細胞之ETIP。於具體實施例中，該ETIP協助自構思(ideation)至開發階段之基因候選者(candidates)及植物表現載體的測試。
