Aspects of the disclosure relate to nucleic acid vaccines. The vaccines include one or more RNA polynucleotides having an open reading frame encoding one or more Chikungunya antigen(s), one or more Zika virus antigens, and one or more Dengue antigens. Methods for preparing and using such vaccines are also described.本發明之態樣係關於核酸疫苗。該等疫苗包括一或多種具有編碼一或多種基孔肯雅抗原(Chikungunya antigen)、一或多種茲卡病毒抗原(Zika virus antigen)及一或多種登革抗原(Dengue antigen)之開放閱讀框架之RNA聚核苷酸。亦描述用於製備及使用該等疫苗之方法。