Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) (RU)
发明人:
Дейген Ирина Михайловна (RU),Кудряшова Елена Вадимовна (RU)
申请号：
RU2015102945
公开号：
RU2015102945A
申请日:
2015.01.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A conjugate of chitosan to stabilize liposome suspensions, characterized in that it is a composition of formula (COHNH) (COHNHX), wherein as a substituent at the amino group X stands PEG or folic acid, where m and n - the number of units in the molecule. 2. The conjugate of claim. 1, characterized in that chitosan is used with a molecular weight of from 15 to 120 tysyach Dalton.3. The conjugate of claim. 1, characterized in that the m / n ratio is from 6 to 19.4. Means for delivering an active agent comprising a base, which is used as a liposomal suspension, noncovalently associated in a complex of chitosan on item 1.5. Means to claim 4, characterized in that in the liposome suspension osnovomolnoe phosphate ratio of lipid on the surface of liposomes to nemoditsifirovannym amino groups is from 1:. 0.25 to 1: 20.6. A method of producing a conjugate according to claim chitosan. 1, comprising preparing a solution of the modifying agent of the amino group of chitosan, mixing the prepared solution with an acidic solution of chitosan and adjusting the resulting mixture to pH 7,5-8,0, followed by purification by dialysis against the buffer rastvora.7. A method according to claim. 6, characterized in that an agent solution, the modifying amino chitosan solution using N-hydroxysuccinimide ester PEG5000-hemisuccinate in DMF or dimetilsulfokside.8. A method according to claim. 6, characterized in that the modifying agent solution was taken in a concentration of from 0.08M to 0,8M.9. A method according to claim. 6, where the modifying agent is a folic acid solution, preactivated solution karbodiimida.10. A method according to claim. 9, which is used as carbodiimide 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide1. Конъюгат хитозана для стабилизации липосомальных суспензий, характеризующийся тем, что он представляет собой композицию в виде формулы (C6O4H9NH2)m(C6O4H9NHX)n, где в качестве заместителя по аминогруппе X выступает ПЭГ или фолиевая кислота, где m и n - количество з
