A formulation of thermosensitive liposomes and, more specifically, a liposome formulation comprising phospholipids and a surfactant, where liposomes tolerate long-term storage at temperatures less than -or equal to -8 ° C, approximately； control the formation of gradients to maximize the potency of the product and release its content at moderately hyperthermic temperatures. The methods for preparing the formulations are also described. Claim 1: A liposomal preparation, comprising a liposome suspension having a gel phase lipid bilayer and an active agent trapped within the liposomes；The fatty bicarbonate includes: (1) one or more diphenyl phosphate selected from phosphoric acid hills, phosphoric acid glycerin, phosphoric acid inoitoles and phosphoric acid ethanol group； (2) one or more diphenyl phosphate made from hydrazine polymer； Y (III) one or more smooth, selected from a group consisting of calcium monophosphate hills, calcium monophosphate glycerin, calcium monophosphate well silicon, and calcium monophosphate ethanolamine； provides the composition of lipid carbonate in an abrasive ratio of about 80-90:2-8:2-18； size in abrasive ratio The lipid weight of the suspension is about 50-150 nm；and where the relative concentration of impurity A after 6 months of storage at a temperature less than -or equal to -8 ° C is less than 0.5%,A is the peak of relative retention time about 1.4 in high performance liquid chromatography (HPLC), and its reverse column is C18. The gradient eludes the acetic acid / methane solvent. 13. Claim 2: the parent preparation of claim 1, in which tubulina is the active agent. 150. Claim 17: the pharmaceutical composition of claim 2, wherein the liposome further comprises an imaging agent or a diagnostic agent.Una formulación de liposomas termosensibles y, más específicamente, a una formulación de liposomas que comprende fosfolípidos y un agente tensioactivo, donde los liposomas toleran el almacenamiento a largo plazo a temperaturas menores
