The present invention relates to a complex culturing method of Inonotus obliquus, Phellinus linteus and Ganoderma lucidum using mushroom extract. Mass cultivation of complex culture mycelia of Inonotus obliquus, Phellinus linteus and Ganoderma lucidum is possible by using the complex culturing method according to the present invention. The complex culture mycelia of the present invention cultured by using the method are conformed to contain beta-glucan or AHCC in a large amount. The complex culture mycelia cultured by the method are able to be easily cultivated in various media in various conditions such as mushroom extract, cereal crops media, porcine placenta media, sea algae media, etc. Further, the cultivation period is shortened in subculture of the complex culture mycelia, compared to the complex culture mycelia manufactured by the conventional method, and the productivity of the complex culture mycelia can be drastically enhanced as the pollution level is low in cultivation. [Reference numerals] (AA) (First process) Step of manufacturing each mushroom pulverized material by pulverizing nonotus obliquus, Phellinus linteus and Ganoderma lucidum, respectively； (BB) (Second process) Step of manufacturing each mushroom extract by respectively extracting nonotus obliquus, Phellinus linteus and Ganoderma lucidum separately prepared from first process with hot water； (CC) (Third process) Step of mixing mushroom pulverized material manufactured in first process with same kind of mushroom extract manufactured in second step, and cultivating； (DD) (Fourth step) Step of mixing mushroom culture of nonotus obliquus, Phellinus linteus and Ganoderma lucidum cultivated third step, and cultivating본 발명은 버섯 추출물을 이용한 차가버섯, 상황버섯 및 영지버섯의 복합배양방법에 관한 것으로서, 본 발명에 따른 복합배양법을 이용하여 차가버섯, 상황버섯 및 영지버섯 균사체의 복합배양균사체를 대량 생산할 수 있다. 상기 방법을 이용해 배양된 본 발명의 복합배양균사체는, 베타글루칸 또는 AHCC를 다량으로 함유하는 것으로 확인된다. 상기 방법을 통해 배양된 복합배양균사체는 버섯 추출물, 곡물배지, 돈태반배지, 해조류배지 등의 각종 조건의 다양한 배지에서도 용이하게 배양가능하다. 또한, 상기 복합배양균사체의
