Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) (RU)
发明人:
Демиденко Наталья Владленовна (RU),Пенин Алексей Александрович (RU)
申请号：
RU2012153913/10
公开号：
RU2012153913A
申请日:
2012.12.13
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method of screening samples buckwheat plant breeding comprising: growing selectable and control populations under normal conditions, followed by placing the sample portion of each population to stress conditions collecting tissue samples of plants and selectable control population exposed and subjected to stress effect determining in the collected samples expression levels of previously identified genes, the level of marker response to the analyzed type of stress comparing gene expression levels in samples placed in stressful conditions and samples grown under normal conditions selection of those specimens in which there is a maximum level change gene expression level marker response to the analyzed type of stress compared to the control populyatsiey.2. A method according to claim 1, characterized in that the identification of genes, marker levels in response type of stress analyzed includes: growing variety of buckwheat samples under normal conditions, followed by placing the samples in the various parts of stress conditions collecting tissue samples of plants subjected to and not subjected to stress factors analysis of the expression level of all genes in the exposed and unexposed samples of the stress effects comparing gene expression levels in samples subjected to various types of stress identifying genes specifically altering the expression level in response to the analyzed type stressa.3. A method according to claim 1, characterized in that the selected selectable samples of the maximum change gene expression level, level of marker response to analyte whith1. Способ отбора селекционных образцов растений гречихи, включающий: выращивание селектируемой и контрольной популяций при нормальных условиях с последующим помещением части образцов каждой популяции в стрессовые условия сбор образцов ткани растений селектируемой и контрольной популяции подвергнутых и не подвергнутых стрессовому воздействию определение в собранных образцах уровней экспрессии предварител
