The enzyme, network of the giant molecule which the constituent of the polymer and the building a bridge medicine shares joins is formed. When the enzyme product which covalent binding it is fixed has enzyme activity, is dried and is retained under ambient conditions, containment the activity which is stabilized. The manner which produces the immobilized enzyme and the manner which uses the said enzyme are disclosed.< Choice figure >Drawing 1酵素、ポリマー及び架橋剤の組成物が共有結合した巨大分子のネットワークを形成する。共有結合的に固定化した酵素調製物は酵素活性を有し、周囲条件で乾燥及び保存される場合、安定した活性を保持する。固定化酵素を製造する方法及び該酵素を使用する方法が開示される。【選択図】図1