Methods of producing neutrophil-depleted platelet-rich plasma by passing a blood, platelet or platelet-rich plasma fraction through a narrow, twisted and/or charged environment to remove neutrophils and produce neutrophil-depleted platelet-rich plasma is described. The neutrophil-depleted platelet-rich plasma may be depleted in neutrophils by 75% or more and includes at least 0.5×106 platelets per ml. The pH of the neutrophil-depleted platelet-rich plasma may be adjusted to 7.3-7.5.
