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[学术文献 ] 葫芦科14-3-3（GRF）基因家族的鉴定及进化分析 进入全文
知网
14-3-3蛋白是植物中一类高度保守的蛋白家族,因其特异识别并结合靶蛋白的磷酸化位点而广泛参与植物生长发育和逆境胁迫响应过程。为系统地了解14-3-3(GRF)基因家族在葫芦科作物中的基本特征和进化关系,本研究利用生物信息学从葫芦科7个物种基因组内共鉴定出83个14-3-3(GRF)基因,其中西瓜中有10个、甜瓜中9个、黄瓜中10个、瓠瓜中9个,3个南瓜属物种基因组各15个。理化特性表明14-3-3(GRF)蛋白的分子量约为30 k D,且大都为酸性氨基酸(pI<7.0)。相对于西瓜、甜瓜、黄瓜和瓠瓜,基因组复制事件是3个南瓜属物种内14-3-3(GRF)基因数目增多的主要原因。进化分析显示,葫芦科14-3-3(GRF)基因家族可进一步被划分为ε类和非ε类亚组,且后者的基因结构(外显子数目,内含子相位)较为保守。本研究结果首次完成了14-3-3(GRF)基因家族在葫芦科作物的鉴定、基因结构、基因家族扩增、共线性及进化分析,为更深入研究该家族基因的生物学功能提供参考。
[学术文献 ] Phylogenetic informativeness analyses to clarify past diversification processes in Cucurbitaceae（葫芦科植物系统发育信息分析以阐明过去的多样性过程） 进入全文
Springer Nature Journal
Phylogenomic studies have so far mostly relied on genome skimming or target sequence capture, which suffer from representation bias and can fail to resolve relationships even with hundreds of loci. Here, we explored the potential of phylogenetic informativeness and tree confidence analyses to interpret phylogenomic datasets. We studied Cucurbitaceae because their small genome size allows cost-efficient genome skimming, and many relationships in the family remain controversial, preventing inferences on the evolution of characters such as sexual system or floral morphology. Genome skimming and PCR allowed us to retrieve the plastome, 57 single copy nuclear genes, and the nuclear ribosomal ITS from 29 species representing all but one tribe of Cucurbitaceae. Node support analyses revealed few inter-locus conflicts but a pervasive lack of phylogenetic signal among plastid loci, suggesting a fast divergence of Cucurbitaceae tribes. Data filtering based on phylogenetic informativeness and risk of homoplasy clarified tribe-level relationships, which support two independent evolutions of fringed petals in the family. Our study illustrates how formal analysis of phylogenomic data can increase our understanding of past diversification processes. Our data and results will facilitate the design of well-sampled phylogenomic studies in Cucurbitaceae and related families.
[学术文献 ] Thermal, Structural and Morphological Studies of Cellulose and Cellulose Nanofibers Extracted from Bitter Watermelon of the Cucurbitaceae Family（从葫芦科苦瓜中提取的纤维素和纤维素纳米纤维的热学、结构和形态研究） 进入全文
Springer Nature Journal
This work presents a new way to prepare and isolate nanometric cellulose fbrils as extracted from Citrullus Colocynthis (Cc) seeds. The nanofbrils were then characterized using spectroscopic, thermal and morphological/structural techniques.IR-Raman spectra of cellulose fbers confrmed that the treatment they went through, efectively removed all non-cellulosic material (i.e. hemicellulose and lignin). In addition to that, TGA results indicated a good and higher thermal stability of the extracted cellulose. HPLC and 1H NMR measurements were carried out to estimate the chemical composition of cellulose from Cc seeds. TEM confrmed the presence of nanofbers in the treated sample; XRD (WAXS-SAXS) data show that the cellulose nanofbers have a higher crystallinity. The preparation (extraction and treatment) of cellulose and cellulose nanofbers from the Cc seeds as well as their thermal, structural and morphological characterizations, were successful.
[学术文献 ] Metabolite Profiling and Quantitation of Cucurbitacins in Cucurbitaceae Plants by Liquid Chromatography coupled to Tandem Mass Spectrometry（采用液相色谱-串联质谱法对葫芦科植物葫芦素的代谢产物进行分析和定量） 进入全文
Springer Nature Journal
Cucurbitaceae is an important plant family because many of its species are consumed as food, and used in herbal medicines, cosmetics, etc. It comprises annual vines and is rich in various bioactive principles which include the cucurbitacins. These steroidal natural products, derived from the triterpene cucurbitane, are mainly the bitter principles of the family Cucurbitaceae. Their biological activities include anti-inflammatory, hepatoprotective, and anti-cancer activities. A total of 10 species belonging to 6 genera of the Cucurbitaceae family along with Cissampelos pareira (Menispermaceae) were included in this study. A comprehensive profiling of certain natural products was developed using HPLC-QTOF-MS/MS analysis and a distribution profile of several major natural products in this family was obtained. A total of 51 natural products were detected in both positive and negative ionization modes, based on accurate masses and fragmentation patterns. Along with this, quantitation of four bioactive cucurbitacins, found in various important plants of the Cucurbitaceae family, was carried out using multiple reaction monitoring (MRM) approach on an ion trap mass spectrometer. Cucurbitacin Q was found to be the most abundant in C. pareira , while Citrullus colocynthis contained all four cucurbitacins in abundant quantities. The developed quantitation method is simple, rapid, and reproducible.
[学术文献 ] Characterization of VQ motif-containing protein family and their expression patterns under phytohormones and abiotic stresses in melon ( Cucumis melo L.)（甜瓜( 瓜蒂 ) VQ motif蛋白家族的特征及其在植物激素和非生物胁迫下的表达模式。） 进入全文
Springer Nature Journal
Many studies have showed plant VQ gene family plays an important part in response to abiotic and biotic stresses. At present, VQ protein family has been found successively in many plants, however, there have no detailed characterization and expression profiles of VQ gene family in muskmelon. Thus, the author probed into genome-wide identification, characterization and expression pattern under various hormones and abiotic stresses. Thirty CmVQ genes were classified ten groups according to orthologous of Arabidopsis VQ genes and dispersedly distributed on 11 chromosomes except for chromosomes 5 and 9. Majority of CmVQ members contained 300 amino acids or less, and were predicted to localize in the nucleus and had no intron. Many cis -elements in response to different stresses and hormones were found in the promoters of CmVQ genes. Gene ontology analysis displayed that 60% CmVQ genes participated in response to stimuli. Quantitative real-time PCR displayed that numerous CmVQ genes showed either positively or negatively regulated expression patterns under hormonal treatments and abiotic stresses. In summary, this research offers a general introduction to melon VQ genes and will contribute to the molecular breeding for resistance to stresses, as well as deeper inquiry on the biological functions of the VQ proteins.
[学术文献 ] 葫芦科园艺作物卷须发生的研究进展 进入全文
维普期刊服务平台
葫芦科Cucurbitaceae植物多为1年生爬藤植物,是农业生产的重要植物科之一。卷须是葫芦科植物关键形态学标记。本研究总结分析了葫芦科植物关于卷须方面的主要研究成果,并对未来的研究提出展望。目前,关于葫芦科园艺作物卷须的研究主要集中于:①卷须的变态来源;②调控卷须发生的关键基因;③卷须组织中特异或高量表达基因;④内源激素调控卷须发育;⑤外界环境对卷须发生的影响。主要结论为:葫芦科园艺作物卷须被确定为侧分枝的变态器官,调控卷须发生的关键基因为TCP1。卷须组织特异或高量表达基因主要参与植物形态建成、趋向性、生长素极性运输、钙离子转运、谷氨酸代谢、木质素代谢等,而这些与卷须发生、发育、卷曲缠绕密切相关。激素(生长素、赤霉素)和外界环境(光、温、水分)均被报道可影响葫芦科园艺作物卷须发育,但具体分子机制均未知。未来葫芦科园艺作物卷须的研究应集中于关键基因TCP1的上下游基因网络解析,完善激素和外界环境对卷须的调控作用。结合日趋成熟的基因编辑技术,探究葫芦科园艺作物卷须的分子调控网络以及开展无卷须育种设计。
